Molecular detection of Anaplasma ovis in small ruminants and ixodid ticks from Mongolia
Molecular detection of Anaplasma ovis in small ruminants and ixodid ticks from Mongolia
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DOI：
10.1016/j.parint.2018.11.004
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发表时间：
2019-04-01
影响因子：
1.9
通讯作者：
Yokoyama, Naoaki
中科院分区：
文献类型：
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作者：
Enkhtaivan, Batsaikhan;Narantsatsral, Sandagdorj;Yokoyama, Naoaki
绵羊无形体是一种蜱传专性细胞内立克次体细菌，可引起家养和野生小型反刍动物无形体病。蒙古绵羊和山羊的总数量约为 4850 万，在该国经济中发挥着至关重要的作用。在这项研究中，我们对蒙古 21 个省中的 19 个省的绵羊和山羊进行了绵羊曲霉的流行病学调查。此外，还对从蒙古 11 个省份收集的未喂食蜱虫中提取的 DNA 样本进行了羊曲霉筛查。通过基于主要表面蛋白 4 基因 (msp4) 的 PCR 检测筛选，在绵羊和山羊的 1179 份和 871 份血液 DNA 样本中，分别有 813 份 (69.0%) 和 621 份 (71.3%) 呈羊曲霉阳性。按省份计算，绵羊和山羊的绵羊绵羊感染率分别为 7.4%-93.3% 和 13.3%-100%。随后，使用相同的 PCR 检测方法从 721 只未喂食的蜱中制备 DNA 样本，其中包括 Nuttalli（n = 378）、persulcatus（n = 95）、Pospelo-vashtromae Haemaphysalis（n = 120）和 Asiaticum（n = 128）。尽管 9 个 D. nuttalli 呈绵羊蜱阳性，但所有其他蜱 DNA 样本均为阴性。除了报告蒙古各地绵羊和山羊体内的绵羊曲霉外，这项研究还确定了 D. nuttalli 是蒙古绵羊曲霉的潜在传播媒介。目前的数据强调了监测蒙古绵羊和山羊可能发生的临床无形体病以及在全国范围内控制纳塔利虫的重要性。
Anaplasma ovis is a tick-borne obligate intracellular rickettsial bacterium that causes anaplasmosis in domestic and wild small ruminants. Sheep and goats, whose combined population is approximately 48.5-million in Mongolia, play a vital role in the country's economy. In this study, we conducted an epidemiological survey of A. ovis in sheep and goats from 19 of 21 provinces in Mongolia. Additionally, DNA samples extracted from unfed ticks collected in 11 Mongolian provinces were also screened for A. ovis. Of 1179 and 871 blood DNA samples from sheep and goats, 813 (69.0%) and 621 (71.3%), respectively, were positive for A. ovis when screened by a PCR assay based on major surface protein 4 gene (msp4). On a per province basis, A. ovis infection rates ranged from 7.4%-93.3% and 13.3%-100% in sheep and goats, respectively. Subsequently, DNA samples prepared from 721 unfed ticks, including Dermacentor nuttalli (n = 378), Ixodes persulcatus (n = 95), Haemaphysalis pospelo-vashtromae (n = 120), and Hyalomma asiaticum (n = 128), were screened for A. ovis using the same PCR assay. Although nine D. nuttalli were A. ovis-positive, all other tick DNA samples were negative. In addition to reporting A. ovis in sheep and goats from all over Mongolia, this study identified D. nuttalli as a potential transmission vector of A. ovis in Mongolia. The present data highlight the importance of monitoring Mongolian sheep and goats for possible episodes of clinical anaplasmosis and controlling D. nuttalli throughout the country.