Glycoproteomics Analysis Reveals Differential Expression of Site-Specific Glycosylation in Human Milk Whey during Lactation
Glycoproteomics Analysis Reveals Differential Expression of Site-Specific Glycosylation in Human Milk Whey during Lactation
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糖蛋白质组学分析揭示了哺乳期间母乳乳清中位点特异性糖基化的差异表达
DOI：
10.1021/acs.jafc.0c07998
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发表时间：
2021
影响因子：
6.1
通讯作者：
Ye Mingliang
中科院分区：
文献类型：
--
作者：
Wang Zhongyu;Zhang Na;Wang Wendan;Li Yitong;Szeto Ignatius M.;Qin Hongqiang;Jin Yan;Ye Mingliang
蛋白质N-糖基化在人乳乳清中起着重要的作用，在婴儿出生后的健康发展。乳清中位点特异性聚糖的变化反映了婴儿在不同情况下的需求。然而，乳清的常规糖蛋白质组学分析不能揭示位点特异性聚糖的变化，因为在分析之前通常将连接的聚糖从糖蛋白中酶促除去。在这项研究中，N-糖蛋白质组学分析的牛奶乳清进行不去除附着的聚糖，并确定了330和327个完整的糖肽在初乳和成熟的牛奶乳清，分别。通过分析鉴定的完整糖肽实现了位点特异性聚糖的无标记定量，其揭示了6个糖位点上的9个显著上调的位点特异性聚糖和8个糖位点上的11个显著下调的位点特异性聚糖。观察到了一些有趣的变化趋势的N-聚糖连接到特定的糖位点在人乳清。在初乳和成熟乳中，发现二等分GlcNAc附着于8种糖蛋白的11个糖位点上。本研究揭示的位点特异性聚糖的动态变化为了解蛋白质N-糖基化在婴儿发育过程中的作用提供了见解。
Protein N-glycosylation in human milk whey plays a substantial role in infant health during postnatal development. Changes in site-specific glycans in milk whey reflect the needs of infants under different circumstances. However, the conventional glycoproteomics analysis of milk whey cannot reveal the changes in site-specific glycans because the attached glycans are typically enzymatically removed from the glycoproteins prior to analysis. In this study, N-glycoproteomics analysis of milk whey was performed without removing the attached glycans, and 330 and 327 intact glycopeptides were identified in colostrum and mature milk whey, respectively. Label-free quantification of site-specific glycans was achieved by analyzing the identified intact glycopeptides, which revealed 9 significantly upregulated site-specific glycans on 6 glycosites and 11 significantly downregulated site-specific glycans on 8 glycosites. Some interesting change trends in N-glycans attached to specific glycosites in human milk whey were observed. Bisecting GlcNAc was found attached to 11 glycosites on 8 glycoproteins in colostrum and mature milk. The dynamic changes in site-specific glycans revealed in this study provide insights into the role of protein N-glycosylation during infant development.