Role of gene body methylation in acclimatization and adaptation in a basal metazoan
Role of gene body methylation in acclimatization and adaptation in a basal metazoan
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DOI：
10.1073/pnas.1813749115
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发表时间：
2018-12-26
影响因子：
11.1
通讯作者：
Matz, Mikhail V.
中科院分区：
文献类型：
--
作者：
Dixon, Groves;Liao, Yi;Matz, Mikhail V.
基因体甲基化（GBM）被假设可以调节对环境变化的反应，包括跨代可塑性，但迄今为止还缺乏证据。在这里，我们表明，珊瑚片段移植到两个遥远的珊瑚礁之间，主要是通过增加或减少全基因组GBM差异：低和高度甲基化基因之间的甲基化水平的范围变得更宽或更窄。值得注意的是，在广泛的功能水平上，这种简单的调整与基因表达变化非常相关，反映了环境响应基因和管家基因表达之间的平衡变化。在我们的实验中，低质量栖息地的珊瑚上调了参与环境反应的基因，而高质量栖息地的珊瑚则更多地投资于管家基因。移植片段显示更接近GBM匹配本地珊瑚获得更高的健身特性，这支持GBM的作用，在驯化。群体之间GBM的固定差异与塑料GBM变化不一致，并且主要在FST升高的基因中观察到，这表明它们主要是通过遗传趋异产生的。然而，我们不能完全排除获得性GBM状态的跨代遗传。
Gene body methylation (GBM) has been hypothesized to modulate responses to environmental change, including transgenerational plasticity, but the evidence thus far has been lacking. Here we show that coral fragments reciprocally transplanted between two distant reefs respond predominantly by increase or decrease in genome-wide GBM disparity: The range of methylation levels between lowly and highly methylated genes becomes either wider or narrower. Remarkably, at a broad functional level this simple adjustment correlated very well with gene expression change, reflecting a shifting balance between expressions of environmentally responsive and housekeeping genes. In our experiment, corals in a lower-quality habitat up-regulated genes involved in environmental responses, while corals in a higher-quality habitat invested more in housekeeping genes. Transplanted fragments showing closer GBM match to local corals attained higher fitness characteristics, which supports GBM's role in acclimatization. Fixed differences in GBM between populations did not align with plastic GBM changes and were mostly observed in genes with elevated FST, which suggests that they arose predominantly through genetic divergence. However, we cannot completely rule out transgenerational inheritance of acquired GBM states.