INCREASE OF PROLACTIN MESSENGER-RNA IN THE RAT HYPOTHALAMUS AFTER INTRACEREBROVENTRICULAR INJECTION OF VIP OR PACAP
INCREASE OF PROLACTIN MESSENGER-RNA IN THE RAT HYPOTHALAMUS AFTER INTRACEREBROVENTRICULAR INJECTION OF VIP OR PACAP
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DOI：
10.1016/0006-8993(94)91303-x
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发表时间：
1994-10-17
期刊：
影响因子：
2.9
通讯作者：
KRUEGER, JM
中科院分区：
文献类型：
--
作者：
BREDOW, S;KACSOH, B;KRUEGER, JM
血管活性肠肽(VIP)、结构同源的垂体腺苷环化酶激活肽(PACAP)和催乳素(PRL)可促进快速眼动睡眠(REMS)。VIP和PACAP都是垂体腺PRL基因表达和释放的诱导剂。关于PRL在大脑中的调节知之甚少，尽管有假说认为I.C.V促进REMS的活动。VIP的给药可能通过激活大脑PRL来实现。为了测试VIP或PACAP是否真的增加了脑内的mRNA，我们将VIP和PACAP的多肽(VIP：30或300pmol；PACAP：220pmol)静脉注射。在夜幕降临时变成老鼠。1h后，从纯化的下丘脑m RNA中合成出c DNA。用聚合酶链式反应、Southern印迹和杂交分析标准量的催乳素。与B-肌动蛋白mRNA水平相比，VIP和PACAP均以剂量依赖的方式增加PRL mRNA水平，尽管VIP在摩尔基础上更有效。未检测到先前报道的选择性剪接的PRL mRNA(缺少外显子4)。这些数据支持中枢VIP和PACAP的促REMS活性可能是由大脑PRL介导的假说。
Vasoactive intestinal peptide (VIP), the structurally homologous pituitary adenylate cyclase-activating peptide (PACAP) and the pituitary hormone, prolactin (PRL) enhance rapid eye movement sleep (REMS). VIP and PACAP are both inducers of PRL gene expression and release in the pituitary gland. Little is known about PRL regulation in the brain although it is hypothesized that the REMS-promoting activity of i.c.v. administered VIP may be mediated via the activation of cerebral PRL. To test whether VIP or PACAP in fact increase intracerebral mRNA, the peptides (VIP: 30 or 300 pmol; PACAP: 220 pmol) were injected i.c.v. into rats at dark onset. 1 h later, cDNA was synthesized from purified hypothalamic mRNA. Standardized amounts were analysed for PRL using the polymerase chain reaction followed by Southern blotting and hybridization. Compared with B-actin mRNA levels, both VIP and PACAP increased PRL mRNA levels in a dose-dependent fashion though VIP was more effective on a molar basis. The previously reported alternatively spliced PRL mRNA (lacking exon 4) was not detected. The data support the hypothesis that the REMS-promoting activity of central VIP and PACAP might be mediated by cerebral PRL.