Processing pathway for protease B of Saccharomyces cerevisiae.
Processing pathway for protease B of Saccharomyces cerevisiae.
复制标题
酿酒酵母的蛋白酶B的处理途径。
DOI：
10.1083/jcb.108.2.309
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发表时间：
1989-02
影响因子：
7.8
通讯作者：
Jones, E W
中科院分区：
文献类型：
--
作者：
Moehle, C M;Dixon, C K;Jones, E W
酿酒酵母的液泡蛋白酶B是由PRB 1基因编码的枯草杆菌蛋白酶样蛋白酶。针对合成肽和源自大肠杆菌的PRB 1开放阅读框（ORF）蛋白产生的抗体与来自酵母的真实蛋白酶B交叉反应。通过使用这些抗体，蛋白酶B的翻译后生物合成途径已被阐明。前蛋白酶原B是进入内质网（ER）的76-kD非糖基化前体，在内质网中它接受一个天冬酰胺连接（Asn连接）和数目不详的非Asn连接碳水化合物侧链。大的糖基化中间体在离开ER之前被蛋白水解加工成39 kD形式。在高尔基复合体中，由于天冬酰胺连接的侧链的加工，39 kD形式变为40 kD。40-kD蛋白酶原B的羧基末端经过蛋白酶A介导的加工成37-kD中间体，其又被快速加工成31-kD成熟蛋白酶B.最终的加工步骤去除了含有Asn连接链的肽;成熟的PrB只有非Asn连接的碳水化合物。
The vacuolar protease B of Saccharomyces cerevisiae is a subtilisin- like protease encoded by the PRB1 gene. Antibodies raised against a synthetic peptide and an Escherichia coli-derived PRB1 open reading frame (ORF) protein cross-react with authentic protease B from yeast. By using these antibodies, the posttranslational biosynthetic pathway of protease B has been elucidated. Preproprotease B is a 76-kD unglycosylated precursor that enters the endoplasmic reticulum (ER), where it receives one asparagine-linked (Asn-linked) and an undetermined number of non-Asn-linked carbohydrate side chains. The large glycosylated intermediate is proteolytically processed to a 39-kD form before exiting the ER. In the Golgi complex, the 39-kD form becomes 40 kD, due to elaboration of the Asn-linked side chain. The carboxyterminal end of the 40-kD proprotease B undergoes protease A- mediated processing to a 37-kD intermediate, which in turn is quickly processed to 31-kD mature protease B. The ultimate processing step removes a peptide containing the Asn-linked chain; mature PrB has only non-Asn-linked carbohydrate.