C-reactive protein from dried blood spots: Application to household air pollution field studies.
C-reactive protein from dried blood spots: Application to household air pollution field studies.
复制标题
干血斑中的 C 反应蛋白：在家庭空气污染现场研究中的应用。
DOI：
10.1111/ina.12603
复制
发表时间：
2020
期刊：
影响因子：
5.8
通讯作者：
Clark,MaggieL
中科院分区：
文献类型：
--
作者：
Young,BonnieN;Peel,JenniferL;Nelson,TracyL;Bachand,AnnetteM;Heiderscheidt,JudyM;Luna,Bevin;Reynolds,StephenJ;Koehler,KirstenA;Volckens,John;Diaz-Sanchez,David;Neas,LucasM;Clark,MaggieL
家庭空气污染(HAP)被认为是心血管疾病的重要危险因素，但临床证据很少，在资源匮乏的环境中很难收集疾病风险的生物标志物。在54名使用烧柴炉灶的尼加拉瓜妇女中，我们评估了48小时HAP(如细颗粒物，PM2.5)和C反应蛋白(CRP)通过干血斑点的横断面相关性；二次分析包括另外7个全身损伤和炎症的生物标记物。我们进行了子研究，以计算尼加拉瓜连续四天收集的生物标记物的组内相关系数(ICC)，并通过计算与科罗拉多州静脉采集的配对样本的相关性来评估测量干血中生物标记物的有效性。HAP的测量与CRP相关(例如，室内PM2.5增加25%与CRP增加7.4%相关[95%可信区间：0.7，14.5])。在4天的时间里，C反应蛋白浓度的大部分变异性是人与人之间的(ICC0.88)，而C反应蛋白浓度在配对的干血和静脉抽取的血清之间高度相关(Spearmanρ=9.96)。二级生物标记物的结果与零关联基本一致，子研究的ICCs和相关性较低。通过干血斑点评估CRP为阐明HAP与心血管疾病风险之间的关系提供了一种可行的方法。
Household air pollution (HAP) is estimated to be an important risk factor for cardiovascular disease, but little clinical evidence exists and collecting biomarkers of disease risk is difficult in low‐resource settings. Among 54 Nicaraguan women with woodburning cookstoves, we evaluated cross‐sectional associations between 48‐hour measures of HAP (eg, fine particulate matter, PM2.5) and C‐reactive protein (CRP) via dried blood spots; secondary analyses included seven additional biomarkers of systemic injury and inflammation. We conducted sub‐studies to calculate the intraclass correlation coefficient (ICC) in biomarkers collected over four consecutive days in Nicaragua and to assess the validity of measuring biomarkers in dried blood by calculating the correlation with paired venous‐drawn samples in Colorado. Measures of HAP were associated with CRP (eg, a 25% increase in indoor PM2.5was associated with a 7.4% increase in CRP [95% confidence interval: 0.7, 14.5]). Most of the variability in CRP concentrations over the 4‐day period was between‐person (ICC: 0.88), and CRP concentrations were highly correlated between paired dried blood and venous‐drawn serum (Spearmanρ= .96). Results for secondary biomarkers were primarily consistent with null associations, and the sub‐study ICCs and correlations were lower. Assessing CRP via dried blood spots provides a feasible approach to elucidate the association between HAP and cardiovascular disease risk.