The mRNA of claudins is expressed in the endolymphatic sac epithelia.
The mRNA of claudins is expressed in the endolymphatic sac epithelia.
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DOI：
10.1016/j.anl.2011.07.004
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发表时间：
2012-08
期刊：
影响因子：
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通讯作者：
Ai Matsubara;T. Miyashita;Terushige Mori;Kosuke Akiyama;Ryuhei Inamoto;Nozomu Mori
中科院分区：
文献类型：
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作者：
Ai Matsubara;T. Miyashita;Terushige Mori;Kosuke Akiyama;Ryuhei Inamoto;Nozomu Mori
阿片类药物是定位于紧密连接（TJ）的膜蛋白家族。最近的研究表明，claudins可以在TJ中形成离子孔，并调节上皮细胞旁离子转运的通透性。内淋巴囊（ES）是内耳的一部分，吸收内淋巴液。ES功能障碍可导致内淋巴积水。在这项研究中，我们专注于细胞旁运输和研究claudin mRNA的表达在ES epitheliates.MATERIALS和METHODS总RNA分离从整个ES上皮细胞的大鼠激光捕获显微切割。RT-PCR检测细胞内claudins的表达。结果SRT-PCR显示cldn 2、cldn 4、cldn 6、cldn 7、cldn 9、cldn 11、cldn 12和cldn 14在大鼠ES上皮细胞中均有表达。这些claudin mRNA的表达在大鼠ES上皮细胞中的证实，通过原位杂交。CONCLUSION我们证明了多种claudin mRNA的表达在大鼠ES上皮细胞。ES上皮细胞中的这些结果与claudins在细胞旁离子转运中的作用一致。
OBJECTIVEClaudins are a family of membrane proteins which localize to tight junctions (TJs). Recent studies have shown that claudins can form pores for ions in the TJs and regulate the permeability of epithelial paracellular ion transport. The endolymphatic sac (ES) is a part of the inner ear, absorbing the endolymphatic fluid. ES dysfunction may result in endolymphatic hydrops. In this study, we focused on the paracellular transport and examined claudin mRNA expression in the ES epithelia.MATERIALS AND METHODSTotal RNA was isolated from whole ES epithelia of rats by laser capture microdissection. RT-PCR was used to evaluate the expression of claudins. The expression of each claudin mRNA in the epithelial cells of rat ES was confirmed by in situ hybridization.RESULTSRT-PCR indicated the expression of cldn2, cldn4, cldn6, cldn7, cldn9, cldn11, cldn12, and cldn14. The expression of these claudin mRNAs in the epithelial cells of rat ES was confirmed by in situ hybridization.CONCLUSIONWe demonstrated mRNA expression of multiple claudins in the rat ES epithelia. These results in the ES epithelia were consistent with a role of claudins in paracellular ion transport.