Soy Peptides Enhance Heterologous Membrane Protein Productivity during the Exponential Growth Phase of Saccharomyces cerevisiae
Soy Peptides Enhance Heterologous Membrane Protein Productivity during the Exponential Growth Phase of Saccharomyces cerevisiae
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DOI：
10.1271/bbb.110965
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发表时间：
2012-03-01
影响因子：
1.6
通讯作者：
Kawarasaki, Yasuaki
中科院分区：
文献类型：
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作者：
Ito, Keisuke;Hikida, Aya;Kawarasaki, Yasuaki
在这项研究中，生产的8个G蛋白偶联受体的酿酒酵母进行了比较，使用两种类型的媒体，其中之一含有大豆肽和其他游离氨基酸。酵母细胞的生长与大豆肽的培养基中得到改善，和6个受体的表达水平增加，在指数期的平均2.3倍，对游离氨基酸为基础的培养基。大豆肽对蛋白质表达的增强可以通过减轻由于异源蛋白质表达引起的氨基酸源耗尽引起的代谢物应激来解释。
In this study, the production of eight G protein-coupled receptors by Saccharomyces cerevisiae was compared using two types of media, one of which contained soy peptides and the other free amino acids. Yeast cell growth improved in the medium with soy peptides, and the expression levels of six of the receptors increased during the exponential phase by an average of 2.3-fold as against the free amino acid-based medium. The enhancement of protein expression by soy peptides can be explained by alleviation of metabolite stress due to amino acid source depletion caused by heterologous protein expression.