Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana
Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana
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DOI：
10.1007/s00425-004-1311-9
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发表时间：
2004-10-01
期刊：
影响因子：
4.3
通讯作者：
Gleeson, PA
中科院分区：
文献类型：
--
作者：
Gilson, PR;Vergara, CE;Gleeson, PA
最近在动物和真菌中描述了一个高尔基体定位的分子家族，该家族具有广泛的卷曲区域和一个短的(类似于40个残基)保守的C-末端结构域，称为GRIP结构域，负责它们在这个细胞器中的位置。利用模式植物拟南芥，我们鉴定了一个编码GRIP蛋白的基因(AtGRIP)。我们证明了AtGRIP的C-末端结构域在植物细胞中作为高尔基体靶向序列发挥作用。在表达AtGRIP的活细胞中的定位研究表明，融合到DsRed2荧光探针上的AtGRIP在转化的烟草原生质体中与高尔基标记α-甘露糖苷酶I广泛共定位。在水稻、玉米、小麦和紫花苜蓿的基因组数据库中也发现了GRIP类序列，这表明该结构域可能是免疫定位研究中有用的高尔基体标记。尽管GRIP结构域之间的序列同源性很低，但植物GRIP序列能够靶向哺乳动物细胞的高尔基体。综上所述，这些数据表明，GRIP结构域蛋白可能参与了在真核生物中保守的靶向机制。
A family of Golgi-localised molecules was recently described in animals and fungi possessing extensive coiled regions and a short (similar to40 residues) conserved C-terminal domain, called the GRIP domain, which is responsible for their location to this organelle. Using the model plant Arabidopsis thaliana, we identified a gene (AtGRIP) encoding a putative GRIP protein. We demonstrated that the C-terminal domain from AtGRIP functions as a Golgi-targeting sequence in plant cells. Localisation studies in living cells expressing the AtGRIP fused to a DsRed2 fluorescent probe, showed extensive co-location with the Golgi marker alpha-mannosidase I in transformed tobacco protoplasts. GRIP-like sequences were also found in genomic databases of rice, maize, wheat and alfalfa, suggesting that this domain may be a useful Golgi marker for immunolocalisation studies. Despite low sequence identity amongst GRIP domains, the plant GRIP sequence was able to target to the Golgi of mammalian cells. Taken together, these data indicate that GRIP domain proteins might be implicated in a targeting mechanism that is conserved amongst eukaryotes.