Beneficial Effects of Korean Red Ginseng in the Progression of Non-Alcoholic Steatohepatitis via FABP4 Modulation
Beneficial Effects of Korean Red Ginseng in the Progression of Non-Alcoholic Steatohepatitis via FABP4 Modulation
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DOI：
10.1142/s0192415x18500817
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发表时间：
2018-01-01
影响因子：
5.7
通讯作者：
Kim, Bumseok
中科院分区：
文献类型：
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作者：
Jeong, Hyeneui;Kim, Jong-Won;Kim, Bumseok
韩国红参（KRG）是一种传统的草药，用于预防多种老年疾病，因为它对代谢紊乱有治疗作用，包括2型糖尿病和脂肪肝。在这项研究中，我们研究了KRG对小鼠非酒精性脂肪性肝炎（NASH）进展的影响。NASH通过分别喂养蛋氨酸和胆碱缺乏的高脂肪或高脂肪/高蔗糖饮食6周或13周来诱导。每个饲料组还经口给予生理盐水（G 0组）或KRG提取物（100、200或400 mg/kg/天;分别为G1、G2和G4组）。KRG在饮食诱导的NASH模型中表现出抗炎和抗纤维化作用。此外，在两个饮食诱导的NASH组中，脂质代谢相关基因的表达水平均显著降低。我们接下来证实了肝脏中FABP 4的表达水平及其调节炎症和/或氧化应激的能力。我们观察到KRG治疗组中FABP 4 mRNA和蛋白水平降低，表明KRG通过调节FABP 4表达影响NASH相关炎症反应的发病机制。体外实验的结果显示在用KRG处理的细胞中的类似模式，表明KRG处理调节FABP 4的表达并随后减少NASH相关的炎症。我们的研究结果表明KRG通过调节肝脏中FABP 4的表达在NASH相关的炎症反应中发挥新的作用。KRG可能是预防NASH进展的安全替代疗法。
Korean red ginseng (KRG) is a traditional herbal medicine used to prevent several geriatric diseases due to its therapeutic effects on metabolic disorder, including type 2 diabetes and fatty liver disease. In this study, we investigated the effects of KRG on the progression of nonalcoholic steatohepatitis (NASH) in mice. NASH was induced by feeding a methionine-and choline-deficient high-fat or high-fat/high-sucrose diet for 6 or 13 weeks, respectively. Each diet group was also orally administered saline (group G0) or KRG extract (100, 200, or 400 mg/kg/day; groups G1, G2, and G4, respectively). KRG showed anti-inflammatory and antifibrogenic effects in the diet-induced NASH models. Furthermore, the expression levels of lipid metabolism-related genes were markedly decreased with KRG treatment in both diet-induced NASH groups. We next confirmed the expression levels of FABP4 in the liver and its ability to regulate inflammation and/or oxidative stress. We observed decreased levels of FABP4 mRNA and protein in the KRG-treated groups indicating that KRG affects the pathogenesis of NASH-related inflammatory responses by modulating FABP4 expression. Results of in vitro experiments showed similar patterns in cells treated with KRG, indicating that KRG treatment regulates the expression of FABP4 and subsequently reduces NASH related inflammation. Our findings suggest a novel role of KRG in NASH-related inflammatory responses via modulation of FABP4 expression in the liver. KRG may be a safe alternative therapy to prevent NASH progression.