Nucleus accumbens subregions:: hodological and immunohistochemical study in the domestic chick (Gallus domesticus)
Nucleus accumbens subregions:: hodological and immunohistochemical study in the domestic chick (Gallus domesticus)
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DOI：
10.1007/s00441-006-0295-0
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发表时间：
2007-02-01
影响因子：
3.6
通讯作者：
Csillag, Andras
中科院分区：
文献类型：
--
作者：
Balint, Eszter;Csillag, Andras
伏隔核是很早以前在鸟类中发现的。然而，这个核的精确定位和范围仍然是一个有争议的问题。我们使用了针对calbindin、神经肽Y和DARPP-32（多巴胺和腺苷相关磷酸化蛋白，32 kDa）的免疫标记来选择性标记假定的伏隔核分支，并跟踪了将生物素化的葡聚糖胺注射到7日龄家鸡的孤束核区域的顺行运输。伏隔核延伸于背侧寰椎座标a10.6和a8.8之间，可细分为核和壳，核对应于腹内侧纹状体和室旁侧侧纹状体与终纹床核，壳代表位于核腹侧和腹外侧的拱形区域。对钙结合蛋白和神经肽Y的免疫反应性在壳细胞中比在核细胞中更强烈。DARPP-32免疫标记在两个分支中没有差异，但在终纹床核中明显较弱，使该核与周围伏隔亚分支分离。来自孤核的纤维主要终止于壳分裂，类似于哺乳动物的情况。虽然核心完全位于内侧纹状体的边界内，但外壳似乎部分重叠在腹侧苍白球上。我们无法用本研究中采用的方法将伏隔体吻侧至a10.6的剩余部分细分为假定的壳和核。这个区域可能与伏隔核的吻极相对应。
The nucleus accumbens was identified in avian species some time ago. However, the precise localization and extent of this nucleus is still a matter of controversy. We have used immunolabeling against calbindin, neuropeptide Y, and DARPP-32 (dopamine- and adenosine-related phosphoprotein, 32 kDa) for the selective marking of putative accumbens subdivisions and have followed the anterograde transport of biotinylated dextran amine injected to the nucleus tractus solitarii region of 7-day-old domestic chicks. The nucleus accumbens extending between rostrocaudal atlas coordinates A 10.6 and A 8.8 can be subdivided into the core and shell, the core corresponding to the ventromedial and juxtaventricular medial striatum laterodorsal to the bed nucleus of stria terminalis, and the shell representing an arched region situated ventrally and ventrolaterally to the core. Immunoreactivity to both calbindin and neuropeptide Y is more intense in the shell than in the core division. DARPP-32 immunolabeling does not differ in the two divisions but is markedly weaker in the bed nucleus of stria terminalis, enabling the separation of this nucleus from the surrounding accumbens subdivisions. Fibers from the nucleus solitarius predominantly terminate in the shell division, similar to the situation described in mammals. Whereas the suggested core lies entirely within the boundary of the medial striatum, the shell seems partially to overlap the ventral pallidum. We have been unable to subdivide the remaining part of accumbens lying rostral to A 10.6 into a putative shell and core by the methods employed in the present study. This region probably corresponds to the rostral pole of the nucleus accumbens.