Fluorimetric determination of aluminium in serum.
Fluorimetric determination of aluminium in serum.
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荧光法测定血清中的铝。
DOI：
10.1039/an9891400839
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
T. Kamiki
中科院分区：
文献类型：
--
作者：
Y. Suzuki;S. Imai;T. Kamiki
本文提出了一种测定血清中铝的新方法--荧光镓试剂[4-氯-3-（2，4-二羟基苯偶氮）-2-羟基苯-1-磺酸]。血清脱蛋白过程中铝的损失通过用20或30%m/V三氯乙酸（TCA）和5%m/VTCA的组合处理来防止。通过用Capriquat（甲基三辛铵氯化物）萃取到氯仿中，形成Fe 3 +-Lumogallion-capriquat三元复合物，从而除去铁（III）。用硫代硫酸盐作掩蔽剂消除了Cu ~（2+）的干扰。检测限为3.6 ng ml-1和校准图是线性高达1.4微克ml-1的铝。使用所提出的方法，铝在健康受试者的血清中的平均浓度被认为是6.8 ng ml-1，与文献中报道的值一致。
A convenient fluorimetric method for the routine determination of aluminium in serum has been developed using lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid]. Losses of aluminium during deproteinisation of the serum were prevented by treatment with a combination of 20 or 30% m/V trichloroacetic acid (TCA) and 5% m/VTCA. Iron(III) was removed by extraction into chloroform with capriquat (methyltrioctylammonium chloride) as an Fe3+-lumogallion-capriquat ternary complex. The interference from Cu2+ was eliminated by using thiosulphate as a masking agent. The detection limit was 3.6 ng ml-1 and the calibration graph was linear up to 1.4 micrograms ml-1 of aluminium. Using the proposed method, the average concentration of aluminium in the serum of healthy subjects was found to be 6.8 ng ml-1, in agreement with values reported in the literature.