Disruption of Nfic Causes Dissociation of Odontoblasts by Interfering With the Formation of Intercellular Junctions and Aberrant Odontoblast Differentiation
Disruption of Nfic Causes Dissociation of Odontoblasts by Interfering With the Formation of Intercellular Junctions and Aberrant Odontoblast Differentiation
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DOI：
10.1369/jhc.2009.952622
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发表时间：
2009-05-01
影响因子：
3.2
通讯作者：
Park, Joo-Cheol
中科院分区：
文献类型：
--
作者：
Lee, Tae-Yeon;Lee, Dong-Seol;Park, Joo-Cheol
我们以前报道过nfic缺陷小鼠表现出短而异常的臼齿根和严重变形的门牙。本研究的目的是利用形态学、免疫组化和RT-PCR分析来解决这些变化的机制。nfic缺陷小鼠在臼齿根和类似门牙的唇冠中表现出异常的成牙细胞和异常的牙本质形成。在这些异常成牙细胞中观察到的最显著的变化是细胞间连接的丧失和ZO-1和occludin的表达降低。结果，它们被分离，呈圆形，失去了细胞的极性和细胞的排列。此外，分离的成牙细胞被困在类似牙本质的矿化组织中，在整体形态上类似于骨牙本质。这些发现表明，Nfic基因的缺失会干扰细胞间连接的形成，从而导致异常的成牙本质分化和异常的牙本质形成。总的来说，这些成牙细胞的变化促进了nfic缺乏小鼠的臼齿短根和异常根的发育。（journal of histochemistry Cytochem, 2009）
We reported previously that Nfic-deficient mice exhibit short and abnormal molar roots and severely deformed incisors. The objective of this study is to address the mechanisms responsible for these changes using morphological, IHC, and RT-PCR analysis. Nfic-deficient mice exhibited aberrant odontoblasts and abnormal dentin formation in molar roots and the labial crown analog of incisors. The most striking changes observed in these aberrant odontoblasts were the loss of intercellular junctions and the decreased expression of ZO-1 and occludin. As a result, they became dissociated, had a round shape, and lost their cellular polarity and arrangement as a sheet of cells. Furthermore, the dissociated odontoblasts became trapped in dentin-like mineralized tissue, resembling osteodentin in the overall morphology. These findings suggest that loss of the Nfic gene interferes with the formation of intercellular junctions that causes aberrant odontoblast differentiation and abnormal dentin formation. Collectively, these changes in odontoblasts contributed to development of molars with short and abnormal roots in Nfic-deficient mice. (J Histochem Cytochem 57:469-476, 2009)