DISTINCTIVE TRAITS OF NORMAL AND TUMOR-DERIVED HUMAN MAMMARY EPITHELIAL-CELLS EXPRESSED IN A MEDIUM THAT SUPPORTS LONG-TERM GROWTH OF BOTH CELL-TYPES
DISTINCTIVE TRAITS OF NORMAL AND TUMOR-DERIVED HUMAN MAMMARY EPITHELIAL-CELLS EXPRESSED IN A MEDIUM THAT SUPPORTS LONG-TERM GROWTH OF BOTH CELL-TYPES
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DOI：
10.1073/pnas.86.4.1249
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发表时间：
1989-02-01
影响因子：
11.1
通讯作者：
SAGER, R
中科院分区：
文献类型：
--
作者：
BAND, V;SAGER, R
本发明描述了一种支持人类原代乳腺肿瘤细胞、来自乳房整形手术的正常上皮细胞和乳腺肿瘤细胞系培养的长期生长的介质。肿瘤细胞在形态、生长要求和两个标志物上与正常乳腺上皮细胞不同：HMFG-2表位优先表达在肿瘤细胞上，亲脂性荧光染料罗丹明123优先滞留在肿瘤细胞线粒体中。HMFG-2荧光素偶联抗体的差示荧光可以用作荧光激活细胞分选机中分离正常和肿瘤细胞的基础，就像罗丹明123的差示保留一样。
A medium is described that supports long-term growth in culture of human primary mammary tumor cells, of normal epithelial cells from mammoplasty, and of mammary tumor cell lines. Tumor cells are shown to be distinguishable from normal mammary epithelial cells by morphology, by growth requirements, and by two markers: preferential expression of the HMFG-2 epitope on tumor cells and preferential retention in tumor cell mitochondria of the lipophilic fluorescent dye rhodamine 123. Differential fluorescence of HMFG-2 fluorescein-conjugated antibodies can be used as a basis for separation of normal and tumor cells in a fluorescence-activated cell sorter, as can differential retention of rhodamine 123.