The dynamic Rab11-FIPs
The dynamic Rab11-FIPs
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DOI：
10.1042/bst0371032
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发表时间：
2009-10-01
影响因子：
3.9
通讯作者：
McCaffrey, Mary W.
中科院分区：
文献类型：
--
作者：
Horgan, Conor P.;McCaffrey, Mary W.
Rab 11-FIPs（Rab 11-family interacting proteins;也称为HIPS）构成进化上保守的蛋白质家族，其充当多个Rab和Arf（ADP-核糖基化因子）GTP酶的效应分子。它们最初的特征在于它们通过高度保守的C-末端RBD（Rab 11-binding domain）结合Rab 11亚家族成员的能力。Rab 11与FIPs复合物的晶体结构的解析表明，RBD介导HIP分子的同源二聚化，为Rab 11结合产生两个对称界面，并导致两个HIP和两个Rab 11分子之间形成异源四聚体复合物。FIP蛋白由五个基因编码，并且已经报道了选择性剪接。基于一级结构，NIPS被细分为两类：I类[Rip 11、FIP 2和RCP（Rab-coupling protein）]和II类（FIP 3和FIP 4）。最近的研究已经确定FIPs是调节多种不同膜运输事件的关键参与者。本文综述了Rab 11-FIP的研究进展，并从分子和细胞水平讨论了FIP功能的最新研究成果。
The Rab11-FIPs (Rab11-family interacting proteins; also known as HIPS) constitute an evolutionarily conserved protein family that act as effector molecules for multiple Rab and Arf (ADP-ribosylation factor) GTPases. They were initially characterized by their ability to bind Rab11 subfamily members via a highly-conserved C-terminal RBD (Rab11-binding domain). Resolution of the crystal structure of Rab11 in complex with FIPs revealed that the RBD mediates homodimerization of the HIP molecules, creating two symmetrical interfaces for Rab11 binding and leading to the formation of a heterotetrameric complex between two HIP and two Rab11 molecules. The FIP proteins are encoded by five genes and alternative splicing has been reported. Based on primary structure, the NIPS were subcategorized into two classes: class I [Rip11, FIP2 and RCP (Rab-coupling protein)] and class II (FIP3 and FIP4). Recent studies have identified the FIPs as key players in the regulation of multiple distinct membrane trafficking events. in this mini-review, we summarize the Rab11-FIP field and discuss, at molecular and cellular levels, the recent findings on FIP function.