Ginsenoside Rg1 prevents early diabetic retinopathy via reducing retinal ganglion cell layer and inner nuclear layer cell apoptosis in db/db mice
Ginsenoside Rg1 prevents early diabetic retinopathy via reducing retinal ganglion cell layer and inner nuclear layer cell apoptosis in db/db mice
复制标题
人参皂苷 Rg1 通过减少 db/db 小鼠视网膜神经节细胞层和内核层细胞凋亡来预防早期糖尿病视网膜病变
DOI：
10.21037/atm.2019.12.155
复制
发表时间：
2020-03-01
影响因子：
--
通讯作者：
Sun, Xiaobo
中科院分区：
文献类型：
--
作者：
Gao, Ye;Ji, Yubin;Sun, Xiaobo
背景：糖尿病视网膜病变（diabetic retinopathy，DR）是糖尿病血管并发症，易导致失明.人参皂苷Rg1（Ginsenoside Rg1，GRg1）是人参中的一种主要皂苷，具有抗凋亡作用。测量血糖、血脂和血管通透性，以及评估病理变化和视网膜各层厚度。免疫荧光和Western blot检测视网膜细胞凋亡相关蛋白表达水平。结果：GRg1能有效降低血糖和甘油三酯水平，维持正常视网膜通透性和生理结构。GRg1通过减少细胞凋亡来维持神经节细胞层（GCL）和内核层（INL）的厚度。结论：这些数据有力地表明GRg1通过减少GCL和INL细胞凋亡来预防糖尿病视网膜病变。GRG1可能是一种有前途的早期DR治疗药物。
Background: Diabetic retinopathy (DR), a diabetic vascular complication, is prone to developing into blindness. Ginsenoside Rg1 (GRg1), a major saponin in ginseng, exerts high anti-apoptotic activity.Methods: This study aimed to explore the protective effects of GRg1 against diabetes-induced retinal damage. Measurements of blood glucose, blood lipids and vascular permeability were performed, as well as assessments of pathological changes, and the retinal thickness of each layer. Retinal cell apoptosis related protein expression levels were measured by immunofluorescence and western blot assays.Results: Our data demonstrated that GRg1 effectively reduced blood glucose and triglyceride levels and maintained normal retinal permeability and physiological structure. GRg1 maintained the thickness of the ganglion cell layer (GCL) and the inner nuclear layer (INL) by reducing cell apoptosis.Conclusions: These data strongly indicate that GRg1 prevents diabetic retinal changes by decreasing GCL and INL cell apoptosis. GRg1 may be a promising drug for early DR treatment.