Differential expression of neuronal genes defines subtypes of disseminated neuroblastoma with favorable and unfavorable outcome
Differential expression of neuronal genes defines subtypes of disseminated neuroblastoma with favorable and unfavorable outcome
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DOI：
10.1158/1078-0432.ccr-06-0985
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发表时间：
2006-09-01
影响因子：
11.5
通讯作者：
Berthold, Frank
中科院分区：
文献类型：
--
作者：
Fischer, Matthias;Oberthuer, Andre;Berthold, Frank
Purpose: Identification of molecular characteristics of spontaneously regressing stage IVS and progressing stage IV neuroblastoma to improve discrimination of patients with metastatic disease following favorable and unfavorable clinical courses.Experimental Design: Serial analysis of gene expression profiles were generated from five stage IVS and three stage IV neuroblastoma. Differential expression of candidate genes was evaluated by real-time quantitative reverse transcription-PCR in 76 pretreatment tumor samples (stage IVS n = 27 and stage IV n = 49). Gene expression-based outcome prediction was determined by Prediction Analysis for Microarrays using 38 tumors as a training set and 38 tumors as a test set.Results: Comparison of serial analysis of gene expression profiles from stage IV and IVS neuroblastoma revealed similar to 500 differentially expressed transcripts, Genes related to neuronal differentiation were observed more frequently in stage IVS tumors as determined by associating transcripts to Gene Ontology annotations. Forty-one candidate genes were evaluated by quantitative reverse transcription- PCR and 18 were confirmed to be differentially expressed (P
