Randomly organized lipids and marginally stable proteins: a coupling of weak interactions to optimize membrane signaling.
Randomly organized lipids and marginally stable proteins: a coupling of weak interactions to optimize membrane signaling.
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随机组织的脂质和边缘稳定的蛋白质：弱相互作用的耦合以优化膜信号传导。
DOI：
10.1016/j.bbamem.2014.03.005
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Hinderliter,Anne
中科院分区：
文献类型：
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作者：
Rice,AnneM;Mahling,Ryan;Fealey,MichaelE;Rannikko,Anika;Dunleavy,Katie;Hendrickson,Troy;Lohese,KJean;Kruggel,Spencer;Heiling,Hillary;Harren,Daniel;Sutton,RBryan;Pastor,John;Hinderliter,Anne
真核生物脂类在双层结构中主要受弱协同作用的影响。这些相互作用赋予了膜高度动态和柔韧的特性。膜中含有C2结构域的蛋白质相互作用弱，相互协作，具有高度的构象可塑性。我们认为，脂质和含有C2结构域的蛋白质共享的这一弱能量性和可塑性的特征增强了细胞跨膜传递信息的能力。我们使用信息论来评估模型和肥大细胞膜的信息存储能力，并使用差示扫描量热法、羧基荧光素释放试验和色氨酸荧光来评估蛋白质和膜的稳定性。肥大细胞膜中脂质的分布编码5.6-5.8亿比特的信息。在细胞膜的内小叶中，比外小叶中含有更多的信息。当模型膜的脂类组成和信息含量发生变化时，相关的C2结构域在稳定性和变性方面发生了很大的变化。因此，含有C2结构域的蛋白质对其相关脂类的组成和信息含量非常敏感。综上所述，这些发现表明，通过膜和进入细胞的信号信息流的最大限度是由膜脂和蛋白质近乎随机分布的协作优化的。这篇文章是题为：界面活性多肽和蛋白质特刊的一部分。客座编辑：威廉·C·温利和卡琳娜·赫里斯托娃。
Eukaryotic lipids in a bilayer are dominated by weak cooperative interactions. These interactions impart highly dynamic and pliable properties to the membrane. C2 domain-containing proteins in the membrane also interact weakly and cooperatively giving rise to a high degree of conformational plasticity. We propose that this feature of weak energetics and plasticity shared by lipids and C2 domain-containing proteins enhance a cell's ability to transduce information across the membrane. We explored this hypothesis using information theory to assess the information storage capacity of model and mast cell membranes, as well as differential scanning calorimetry, carboxyfluorescein release assays, and tryptophan fluorescence to assess protein and membrane stability. The distribution of lipids in mast cell membranes encoded 5.6–5.8 bits of information. More information resided in the acyl chains than the head groups and in the inner leaflet of the plasma membrane than the outer leaflet. When the lipid composition and information content of model membranes were varied, the associated C2 domains underwent large changes in stability and denaturation profile. The C2 domain-containing proteins are therefore acutely sensitive to the composition and information content of their associated lipids. Together, these findings suggest that the maximum flow of signaling information through the membrane and into the cell is optimized by the cooperation of near-random distributions of membrane lipids and proteins. This article is part of a Special Issue entitled: Interfacially Active Peptides and Proteins. Guest Editors: William C. Wimley and Kalina Hristova.