Silk protein aggregation kinetics revealed by Rheo-IR
Silk protein aggregation kinetics revealed by Rheo-IR
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DOI：
10.1016/j.actbio.2013.10.032
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发表时间：
2014-02-01
影响因子：
9.7
通讯作者：
Holland, Chris
中科院分区：
文献类型：
--
作者：
Boulet-Audet, Maxime;Terry, Ann E.;Holland, Chris
丝纤维的显著机械性能源于当含水蛋白质“熔体”通过流动转化为不溶性固体时产生的多尺度层次结构。为了直接将丝蛋白的结构和功能与流动反应联系起来，我们首次应用了Rheo-IR平台，该平台将锥板流变学与衰减全反射红外光谱法结合起来。这项技术通过将剪切变稀与分子排列的增加联系起来，为丝蛋白的加工提供了一个新的窗口，剪切变稠影响了丝蛋白二级结构的变化。此外，与其他静态表征方法相比，Rheo-IR被证明在揭示天然（天然）和重组丝原料之间的内在差异方面特别有用。因此，Rheo-IR为天然丝绸加工提供了重要的新见解。这具有内在的学术价值，但在设计与其他聚合物系统（无论是天然的还是合成的）一起使用的重组丝类似物时，它也可能是有用的。(C)2013 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The remarkable mechanical properties of silk fibres stem from a multi-scale hierarchical structure created when an aqueous protein "melt" is converted to an insoluble solid via flow. To directly relate a silk protein's structure and function in response to flow, we present the first application of a Rheo-IR platform, which couples cone and plate rheology with attenuated total reflectance infrared spectroscopy. This technique provides a new window into silk processing by linking shear thinning to an increase in molecular alignment, with shear thickening affecting changes in the silk protein's secondary structure. Additionally, compared to other static characterization methods for silk, Rheo-IR proved particularly useful at revealing the intrinsic difference between natural (native) and reconstituted silk feedstocks. Hence Rheo-IR offers important novel insights into natural silk processing. This has intrinsic academic merit, but it might also be useful when designing reconstituted silk analogues alongside other polymeric systems, whether natural or synthetic. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.