Cancer-Erythrocyte Hybrid Membrane-Camouflaged Magnetic Nanoparticles with Enhanced Photothermal-Immunotherapy for Ovarian Cancer
Cancer-Erythrocyte Hybrid Membrane-Camouflaged Magnetic Nanoparticles with Enhanced Photothermal-Immunotherapy for Ovarian Cancer
复制标题
癌症-红细胞杂化膜伪装磁性纳米粒子增强光热免疫疗法治疗卵巢癌
DOI：
10.1021/acsnano.1c07180
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发表时间：
2021-12-28
期刊：
影响因子：
17.1
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
--
作者：
Xiong, Jiaqiang;Wu, Meng;Zhang, Wei
细胞膜包裹的纳米颗粒由于其固有的细胞特性而被广泛研究，例如免疫逃逸和同源归巢。细胞膜包被还可以通过细胞膜包封技术在复杂血液环境中的循环过程中保持纳米颗粒的相对稳定性。在这项研究中，我们融合了鼠源性ID 8卵巢癌细胞膜与红细胞（RBC）膜，以创建一个混合的仿生涂层（HMF），和混合的仿生吲哚菁绿色（ICG）负载的磁性纳米粒子（Fe 3 O 4-ICG@ HMF）制备卵巢癌的联合治疗。Fe_3 O_4-ICG@pH4保留了ID 8和RBC细胞膜蛋白，并在体外和体内表现出高度特异性的ID 8细胞自我识别以及延长血液循环寿命。有趣的是，在双侧腹侧肿瘤模型中，IRM包被的纳米颗粒也激活了特异性免疫，其杀死同源ID 8肿瘤细胞，但对B16-F10肿瘤细胞没有影响。此外，Fe 3 O 4-ICG@p53具有协同光热效应，通过光热诱导肿瘤坏死释放全细胞肿瘤抗原，通过激活CD 8（+）细胞毒性T细胞和减少调节性Foxp 3（+）T细胞，进一步增强对原发性肿瘤和转移性肿瘤的抗肿瘤免疫治疗。总之，仿生Fe 3 O 4-ICG@ nanoparticles显示协同光热免疫治疗卵巢癌。
Cell-membrane-coated nanoparticles are widely studied due to their inherent cellular properties, such as immune escape and homologous homing. A cell membrane coating can also maintain the relative stability of nanoparticles during circulation in a complex blood environment through cell membrane encapsulation technology. In this study, we fused a murine-derived ID8 ovarian cancer cell membrane with a red blood cell (RBC) membrane to create a hybrid biomimetic coating (IRM), and hybrid IRM camouflaged indocyanine green (ICG)-loaded magnetic nanoparticles (Fe3O4-ICG@IRM) were fabricated for combination therapy of ovarian cancer. Fe3O4-ICG@IRM retained both ID8 and RBC cell membrane proteins and exhibited highly specific self-recognition of ID8 cells in vitro and in vivo as well as a prolonged circulation lifetime in blood. Interestingly, in the bilateral flank tumor model, the IRM-coated nanoparticles also activated specific immunity, which killed homologous ID8 tumor cells but had no effect on B16-F10 tumor cells. Furthermore, Fe3O4-ICG@IRM showed synergistic photothermal therapy, resulting in the release of whole-cell tumor antigens by photothermal-induced tumor necrosis, which further enhanced antitumor immunotherapy for primary tumor and metastatic tumor by activating CD8(+) cytotoxic T cells and reducing regulatory Foxp3(+) T cells. Together, the biomimetic Fe3O4-ICG@IRM nanoparticles showed synergistic photothermal-immunotherapy for ovarian cancer.