Compatibility between Calpurnia aurea leaf extract, attraction aggregation, and attachment pheromone and entomopathogenic fungus Metarhizium anisopliae on viability, growth, and virulence of the pathogen
Compatibility between Calpurnia aurea leaf extract, attraction aggregation, and attachment pheromone and entomopathogenic fungus Metarhizium anisopliae on viability, growth, and virulence of the pathogen
复制标题
Calpurnia aurea 叶提取物、吸引力聚集和附着信息素与昆虫病原真菌绿僵菌 (Metarhizium anisopliae) 之间的相容性对病原体的活力、生长和毒力的影响
DOI：
10.1007/s10340-011-0399-5
复制
发表时间：
2012
影响因子：
4.8
通讯作者：
J. Eloff
中科院分区：
文献类型：
--
作者：
P. Nana;N. K. Maniania;R. Maranga;H. I. Boga;H. Kutima;J. Eloff
绿僵菌Metarhizium anisopliae sensu stricto（ss）（Metsch.）索罗克分离物ICIPE 07正被开发为用于控制蜱的生物杀虫剂。此外，Calpurnia aurea Benth的叶提取物和吸引聚集和附着信息素（AAAP）被用作蜱的引诱剂。这三种药剂被考虑用于自动传播方法中的组合使用，其中被吸引到化学信息素的蜱被接种物感染。因此，进行了实验，以评估在体外的C。aurea、AAAP和M.绿僵菌对营养生长、分生孢子产生和孢子活力的影响。在所有测试浓度下，Calpurnia aurea叶提取物与真菌相容，而AAAP抑制所有真菌生长参数。对M.金龟子用C.的可乳化提取物配制。在实验室生物测定中，还测试了Aurea对不同发育阶段的扇头蜱的作用。在毒力方面，M.单独施用绿僵菌和M.在不同浓度的C.金叶提取物这些结果表明C.金叶提取物与M.绿僵菌和AAAP可以混合使用，作为一种低成本、环境友好的技术来控制放牧场的蜱类，而AAAP应该单独使用。
Metarhizium anisopliae sensu stricto (ss) (Metsch.) Sorok. isolate ICIPE 07 is being developed as biopesticide for the control of ticks. In addition, leaf extracts of Calpurnia aurea Benth, and the attraction aggregation and attachment pheromone (AAAP) are being used as ticks’ attractant. The three agents are being considered for use in combination in an autodissemination approach, whereby ticks that are attracted to semiochemicals are infected with the inoculum. Experiments were therefore conducted to evaluate in vitro the compatibility between C. aurea, AAAP, and the M. anisopliae on vegetative growth, conidial production, and spore viability. Calpurnia aurea leaf extract was compatible with the fungus at all the concentrations tested, whereas AAAP inhibited all the fungal growth parameters. The virulence of M. anisopliae formulated in emulsifiable extracts of C. aurea was also tested against different developmental stages of Rhipicephalus appendiculatus in laboratory bioassays. No significant differences in virulence were observed between M. anisopliae applied alone and M. anisopliae formulated in different concentrations of C. aurea leaf extracts. These results suggest that C. aurea leaf extracts is compatible with M. anisopliae and could be mixed together for “spot-spray” treatments as low-cost and environmental-friendly technology to control ticks in grazing field, while AAAP should be used separately.