A simplified and efficient method for the analysis of fatty acid methyl esters suitable for large clinical studies
A simplified and efficient method for the analysis of fatty acid methyl esters suitable for large clinical studies
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DOI：
10.1194/jlr.d500022-jlr200
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发表时间：
2005-10-01
影响因子：
6.5
通讯作者：
Salem, N
中科院分区：
文献类型：
--
作者：
Masood, A;Stark, KD;Salem, N
用于脂肪酸分析的传统样品制备是一个复杂的多步骤过程，仅气相色谱 (GC) 分析每个样品可能需要 > 1 小时才能解析脂肪酸甲酯 (FAME)。快速 GC 分析适用于人血浆 FAME 分析，使用改进的聚乙二醇色谱柱，该色谱柱具有更小的内径、更薄的固定相膜、更高的载气线速度和更快的升温速度。 Our results indicated that fast GC analyses were comparable to conventional GC in peak resolution.基于 Lepage 和 Roy 的传统酯交换方法被简化为一步法，消除了中和和离心步骤。还开发了一种适合机器人技术的方法，该方法具有较低的甲基化温度，并采用使用多种试剂添加的开管形式。与原始 Lepage 和 Roy 方法相比，简化方法产生的结果在数量上相似且变异系数相似。目前的简化方法适用于人血浆的直接脂肪酸分析，适合研究性研究，并将促进大型临床试验并使群体研究成为可能。
Conventional sample preparation for fatty acid analysis is a complicated, multiple- step process, and gas chromatography ( GC) analysis alone can require > 1 h per sample to resolve fatty acid methyl esters ( FAMEs). Fast GC analysis was adapted to human plasma FAME analysis using a modified polyethylene glycol column with smaller internal diameters, thinner stationary phase films, increased carrier gas linear velocity, and faster temperature ramping. Our results indicated that fast GC analyses were comparable to conventional GC in peak resolution. A conventional transes-terification method based on Lepage and Roy was simplified to a one- step method with the elimination of the neutralization and centrifugation steps. A robotics- amenable method was also developed, with lower methylation temperatures and in an open- tube format using multiple reagent additions. The simplified methods produced results that were quantitatively similar and with similar coefficients of variation as compared with the original Lepage and Roy method. The present streamlined methodology is suitable for the direct fatty acid analysis of human plasma, is appropriate for research studies, and will facilitate large clinical trials and make possible population studies.