Duration of gestation in pregnant dogs carrying cloned fetuses
Duration of gestation in pregnant dogs carrying cloned fetuses
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DOI：
10.1016/j.theriogenology.2012.08.011
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发表时间：
2013-01-15
期刊：
影响因子：
2.8
通讯作者：
Lee, Byeong Chun
中科院分区：
文献类型：
--
作者：
Kim, Min Jung;Oh, Hyun Ju;Lee, Byeong Chun
本研究的目的是探讨妊娠期的持续时间和生理特性的克隆胎儿的怀孕狗，特别是在发情期。进行了一项回顾性研究，以比较妊娠克隆（体细胞核移植）胎儿（克隆组）与非克隆胎儿（对照组）的女性妊娠持续时间，并评估了克隆组中窝仔数、出生体重和体细胞供体品种对妊娠持续时间的影响。同时比较两组产妇临床分娩起始体征与血清孕酮浓度和直肠温度的关系。从排卵日计算的妊娠期克隆组（62.8 ± 0.3天）明显长于对照组（60.9 ± 0.5天; P < 0.001）。两组的窝仔数与妊娠期呈负相关（r = -0.59; P < 0.01），克隆组的出生体重、供体品种与妊娠期无显著差异。尽管对照组和克隆组在发情期的基础直肠温度没有差异，（分别为36.9 +/- 0.1 ℃和37.2 +/- 0.1 ℃），克隆组分娩当天血清孕酮浓度显著高于对照组（2.2 ± 0.4 ng/ml）与对照组（0.5 ± 0.1 ng/ml; P < 0.05）相比。携带克隆胎儿的孕犬妊娠期较长可能是由于该组的窝仔数较少。此外，在克隆狗怀孕的发情期，血清孕酮水平下降较弱，表明分娩信号过程可能会改变，导致妊娠期延长。(C)2013 Elsevier Inc. All rights reserved.
The aim of this study was to investigate gestation duration and the physiologic characteristics of pregnant dogs bearing cloned fetuses, especially in the prepartum period. A retrospective study was performed to compare gestation duration in females pregnant with cloned (somatic cell nuclear transfer) fetuses (cloned group) with those bearing noncloned fetuses (control group), and effects of litter size, birth weight, and breed of somatic cell donors on gestation duration in the cloned group were evaluated. Clinical delivery onset signs associated with serum progesterone concentration and rectal temperature were also compared in both groups. The gestation duration calculated from day of ovulation was significantly longer in the cloned (62.8 +/- 0.3 days) versus the control group (60.9 +/- 0.5 days; P < 0.001). There was a negative correlation between litter size and gestation duration including both groups (r = -0.59; P < 0.01), but there were no differences between birth weights or breed of cell donors and gestation duration in the cloned group. Even though the basal rectal temperature in the prepartum period was not different between control and cloned groups (36.9 +/- 0.1 degrees C and 37.2 +/- 0.1 degrees C, respectively), serum progesterone concentration on delivery day was significantly higher in the cloned group (2.2 +/- 0.4 ng/ml) compared with the control group (0.5 +/- 0.1 ng/ml; P < 0.05). The longer gestation duration of pregnant dogs bearing cloned fetuses might be because of the smaller litter size in this group. Also, the weaker drop in serum progesterone levels in the prepartum period in cloned dog pregnancies indicates that the parturition signaling process might be altered resulting in longer gestation periods. (C) 2013 Elsevier Inc. All rights reserved.