The embryonal carcinoma stem cell Ela-like activity involves a differentiation-regulated transcription factor.
The embryonal carcinoma stem cell Ela-like activity involves a differentiation-regulated transcription factor.
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胚胎癌干细胞Ela样活性涉及分化调节转录因子。
DOI：
10.1093/nar/18.10.2929
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发表时间：
1990
影响因子：
14.9
通讯作者：
Rigby,PW
中科院分区：
文献类型：
--
作者：
LaThangue,NB;Thimmappaya,B;Rigby,PW
小鼠F9胚胎癌（EC）干细胞具有ela样转录活性，这在分化为壁内胚层样细胞（F9- pe）的F9细胞中检测不到。我们使用ela诱导的腺病毒E2A启动子来进一步定义这种活性，我们发现在体外F9 EC和F9- pe细胞提取物中该启动子的转录反映了体内的调节。在EC细胞提取物中，几个反式作用蛋白因子结合到E2A启动子序列上。含有CRE的远端结构域结合F9 EC， F9- pe和Hela细胞提取物中的蛋白质。−71和−50之间的序列定义了多种结合活性，称为DRTF1，所有这些结合活性都在EC干细胞分化过程中下调。DRTF2是一种低丰度的受调控的结合活性，它需要与DRTF1所需的DNA序列重叠。CRE和DRTF1结合位点在体外竞争转录，表明in EC细胞提取了各自的蛋白，作为积极作用的结合位点依赖的转录因子。DRTF1与先前定义的在腺病毒感染期间诱导的HeLa细胞因子E2F的比较表明，尽管这两个因子识别启动子的同一区域，但它们之间存在明显差异。这些数据表明，多种因素对F9 EC细胞提取物中E2A启动子的有效转录是必要的，并表明DRTF1至少在一定程度上负责细胞ela样活性的发育调节。
Murine F9 embryonal carcinoma (EC) stem cells have an Ela-like transcription activity that is undetectable in F9 cells differentiated to parietal endoderm-like cells (F9-PE). The Ela-inducible adenovlrus E2A promoter has been used to further define this activity and we show that in vitro the transcription of this promoter in F9 EC and F9-PE cell extracts reflects the regulationin vivo. In EC cell extracts severaltrans-acting protein factors bind to E2A promoter sequences. A distal domain containing a CRE binds proteins present in F9 EC, F9-PE and Hela cell extracts. Sequences between −71 and −50 define a multiplicity of binding activities, termed DRTF1, all of which are down regulated as EC stem cells differentiate. DRTF2, a low abundance, regulated binding activity requires DNA sequences that overlap those required by DRTF1. The CRE and the DRTF1 binding site compete for transcription in vitro, indicating that In EC cell extracts the respective proteins function as positively acting, binding site dependent transcription factors. Comparison of DRTF1 with the previously defined HeLa cell factor E2F, induced during adenovirus infection, indicates that although both factors recognise the same region of the promoter there are clear differences between them. These data indicate that multiple factors are necessary for efficient transcription of the E2A promoter in F9 EC cell extracts and suggest that DRTF1 is responsible, at least in part, for the developmental regulation of the cellular Ela-like activity.