Stability of microbial functionality in anammox sludge adaptation to various salt concentrations and different salt-adding steps
Stability of microbial functionality in anammox sludge adaptation to various salt concentrations and different salt-adding steps
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DOI：
10.1016/j.envpol.2020.114713
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发表时间：
2020-09-01
影响因子：
8.9
通讯作者：
Wu, Jer-Horng
中科院分区：
文献类型：
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作者：
Naufal, Muhammad;Wu, Jer-Horng
厌氧氨氧化(Anammox)污泥适应不同盐度变化的群落功能的稳定性受到关注，但尚未得到充分探讨。在本研究中，设计了两个厌氧氨氧化反应器，以响应不同的盐度和加盐方式。在盐浓度为0%~4%的范围内，反应器PI以较小的递增盐量(0.5%~1.0%)运行，对进水中90%的亚硝酸盐和氨氮的去除效果较好。相比之下，在盐分急剧增加(2.5%)的情况下运行的反应器SI，在具有新的稳定状态的相同盐分范围内表现出性能降低(高达44%)。观测到的盐扰动后的回弹时间表明，在所有施加的盐水平下，PI反应器基本上和快速地恢复。16S rRNA基因扩增子序列的主坐标分析表明，厌氧氨氧化污泥的细菌群落结构随着盐度的变化发生了明显的变化。然而，定量PCR分析表明，在0.5%-4%的盐度范围内，厌氧氨氧化污泥中编码肼合成酶(HzsA)、细菌和古细菌氨单加氧酶(AmoA)、亚硝酸氧化还原酶(NxrB)、亚硝酸盐还原酶(NirK)和一氧化二氮还原酶(NosZ)的氮转换基因的拷贝数没有显著的变化(p>；0.05)，这表明微生物群落在渗透适应过程中的功能是稳定的。淡水厌氧氨氧化钙。Kuenenia通过潜在地采用高盐分和低SALT-in策略来控制这两个反应堆，从而显示出高渗透适应能力。定量转录本分析表明，在长期暴露于4%盐度的条件下，SI反应器中hzsA转录本所代表的厌氧氨氧化活性细菌数量比PI反应器中的活性厌氧氨氧化细菌数量低约两个数量级，体现了加盐方法的影响。这些结果为Anammox微生物组的渗透适应提供了新的见解，并将有助于管理盐度对脱氮过程的影响。(C)2020爱思唯尔有限公司。保留所有权利。
The stability of community functioning in anaerobic ammonia oxidation (anammox) sludge adaptation to various salinity changes are concerned but not fully explored. In this study, two anammox reactors were designed in response to different salt levels and salt-adding methods. The reactor PI, run with small stepwise salt increments (0.5%-1.0%), removed >90% of nitrite and ammonium in the influent over the range of 0%-4% salt. By contrast, the reactor SI, run with a sharp salt increment (>2.5%), exhibited a reduced performance (by up to 44%) over the same salt range with a new steady state. The observed resilience times after salt perturbations indicated that the PI reactor recovered substantially and rapidly at all imposed salt levels. Principal coordinates analysis of 16S rRNA gene amplicon sequences revealed that bacterial community structures of the anammox sludge altered conspicuously in response to the salinity changes. However, quantitative PCR analysis showed that the shift in copy number of studied nitrogen-converting genes encoding hydrazine synthase (hzsA), bacterial and archaeal ammonia monooxygenases (amoA), nitrite oxidoreductase (nxrB), nitrite reductase (nirK), and nitrous oxide reductase (nosZ) was not significant (p > 0.05) in anammox sludge across the salt levels of 0.5%-4%, which suggests the stability of microbial community functioning in the osmoadaptation processes. The freshwater anammox Ca. Kuenenia showed high osmoadaptation by potentially adopting both high-salt-in and lowsalt-in strategies to dominate in both reactors. The quantitative transcript analysis showed that the active anammox bacteria represented by hzsA transcripts in the SI reactor were approximately two orders of magnitude lower than those in the PI reactor during the long-term exposure to 4% salinity, manifesting the influence by the salt-increasing methods. These results provided new insight into osmo-adaptation of the anammox microbiome and will be useful for managing salinity effects on nitrogen removal processes. (C) 2020 Elsevier Ltd. All rights reserved.