Mapping phosphorylation rate of fluoro-deoxy-glucose in rat brain by 19F chemical shift imaging
Mapping phosphorylation rate of fluoro-deoxy-glucose in rat brain by 19F chemical shift imaging
复制标题
DOI：
10.1016/j.mri.2013.10.015
复制
发表时间：
2014-05-01
影响因子：
2.5
通讯作者：
Hyder, Fahmeed
中科院分区：
文献类型：
--
作者：
Coman, Daniel;Sanganahalli, Basavaraju G.;Hyder, Fahmeed
2-氟-2-脱氧-C-葡萄糖(FDG)和2-氟-2-脱氧葡萄糖-6-磷酸(FDG-6P)的F-19磁共振波谱(MRS)研究可用于直接评估体内总糖代谢。到目前为止，F-19MRS对大脑中FDG磷酸化的测量要么是从提取的组织中获得的，要么是通过异常长的获取时间在体内实现的。电生理和功能磁共振成像(FMRI)测量表明，高达500 mg/kg的FDG剂量可以耐受，对大脑生理和诱发对前爪刺激的Imri-bold反应的副作用最小。在氟烷麻醉的大鼠中，我们用F-19二维化学位移成像(CSI)在11.7T处对FDG和FDG-6P MRS信号进行了定位检测和分离。基于血浆和脑组织之间的可逆传输的代谢模型，包括非饱和血浆到组织成分，用来计算大鼠脑内雾和FDG-6P浓度的空间分布。此外，还估算了雾向FDG-6P转化的速率常数和代谢通量的空间分布。通过F-19 CSI将雾化率映射到FDG-6P转换提供了一种MR方法学，可能会影响其他体内应用，如肿瘤病理生理学的特征。(C)2014 Elsevier Inc.保留所有权利。
F-19 magnetic resonance spectroscopy (MRS) studies of 2-fluoro-2-deoxy-c-glucose (FDG) and 2-fluoro-2-deoxyo-glucose-6-phosphate (FDG-6P) can be used for directly assessing total glucose metabolism in vivo. To date, F-19 MRS measurements of FDG phosphorylation in the brain have either been achieved ex vivo from extracted tissue or in vivo by unusually long acquisition times. Electrophysiological and functional magnetic resonance imaging (fMRI) measurements indicate that FDG doses up to 500 mg,/kg can be tolerated with minimal side effects on cerebral physiology and evoked IMRI-BOLD responses to forepaw stimulation. In halothane-anesthetized rats, we report localized in vivo detection and separation of FDG and FDG-6P MRS signals with F-19 2D chemical shift imaging (CSI) at 11.7 T. A metabolic model based on reversible transport between plasma and brain tissue, which included a non-saturable plasma to tissue component was used to calculate spatial distribution of FOG and FDG-6P concentrations in rat brain. In addition, spatial distribution of rate constants and metabolic fluxes of FOG to FDG-6P conversion conversion were estimated. Mapping the rate of FOG to FDG-6P conversion by F-19 CSI provides an MR methodology that could impact other in vivo applications such as characterization of tumor pathophysiology. (C) 2014 Elsevier Inc. All rights reserved.