Comparing mutation rates under the Luria-Delbruck protocol
Comparing mutation rates under the Luria-Delbruck protocol
复制标题
DOI：
10.1007/s10709-016-9904-3
复制
发表时间：
2016-06-01
期刊：
影响因子：
1.5
通讯作者：
Zheng, Qi
中科院分区：
文献类型：
--
作者：
Zheng, Qi
比较Luria-Delbruck方案下的微生物突变率是一项例行的实验室任务。然而，由于缺乏适当的统计方法，这项重要任务的执行受到了阻碍。目视检查或不当使用t检验和Mann-Whitney检验可能会损害基因研究的质量。本文提出了一个构建似然比检验的统一框架，该检验克服了正确比较微生物突变率的三个重要障碍。具体地说，已经设计了用于似然比检验的算法，该算法允许部分平板化、不同的生长率和不相等的终端细胞种群大小。通过计算机模拟对新算法进行了评估。此外，通过重新分析结核分枝杆菌对抗生素耐药性研究的实验数据，说明了多重比较的策略。
Comparison of microbial mutation rates under the Luria-Delbruck protocol is a routine laboratory task. However, execution of this important task has been hampered by the lack of proper statistical methods. Visual inspection or improper use of the t test and the Mann-Whitney test can impair the quality of genetic research. This paper proposes a unified framework for constructing likelihood ratio tests that overcome three important obstacles to the proper comparison of microbial mutation rates. Specifically, algorithms for likelihood ratio tests have been devised that allow for partial plating, differential growth rates and unequal terminal cell population sizes. The new algorithms were assessed by computer simulations. In addition, a strategy for multiple comparison was illustrated by reanalyzing the experimental data from a study of bacterial resistance against tuberculosis antibiotics.