Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes
Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes
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DOI：
10.1038/ng0197-47
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发表时间：
1997-01-01
期刊：
影响因子：
30.8
通讯作者：
OBrien, SJ
中科院分区：
文献类型：
--
作者：
Lyons, LA;Laughlin, TF;OBrien, SJ
哺乳动物基因图谱的精确比较需要共同的锚定位点作为保守染色体片段的标志。使用计算机脚本自动进行 DNA 序列数据库比对。我们设计了 410 个进化上保守的引物对序列，这些序列对于从任何哺乳动物物种的 DNA 中扩增锚定位点基因具有特异性。引物对被设计为跨越内含子以进行多态性确定。并包含足够的外显子序列（25-400 bp）以进行基因鉴定。总共针对家猫优化了318对引物，测序的猫PCR产物中86%与引物来源的基因具有同源性。对来自 11 个目的 20 只哺乳动物的筛选显示，318 个引物中的 35-52% 无需进一步优化即可产生单一 PCR 产物，这表明近 75% 的引物可以针对任何真兽类哺乳动物进行优化。
Precise comparisons of mammalian gene maps require common anchor loci as landmarks for conserved chromosomal segments. Using a computer script that automates DNA sequence database alignments. we designed 410 evolutionarily conserved primer pair sequences which are specific for anchor locus gene amplification from any mammalian species' DNA. Primer pairs were designed to span introns for polymorphism ascertainment. and to include sufficient exonic sequence (25-400 bp) to allow for gene identification. A total of 318 primer pairs were optimized for domestic cats, and 86% of the sequenced feline PCR products showed homology to the gene of primer origin. A screen of 20 mammals from 11 orders revealed that 35-52% of the 318 primers yielded a single PCR product without further optimization suggesting that nearly 75% can be optimized for any eutherian mammal.