Coaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(ε-caprolactone) nanofibers for sustained release
Coaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(ε-caprolactone) nanofibers for sustained release
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DOI：
10.1021/bm050743i
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发表时间：
2006-04-01
影响因子：
6.2
通讯作者：
Ramakrishna, S
中科院分区：
文献类型：
--
作者：
Zhang, YZ;Wang, X;Ramakrishna, S
纳米纤维是一种具有仿生功能的组织工程支架材料。我们演示了用同轴静电纺丝技术将模型蛋白异硫氰酸荧光素偶联牛血清白蛋白（fitcBSA）与水溶性聚合物聚乙二醇（PEG）一起封装在可生物降解的聚聚己内酯（PCL）纳米纤维中。内流速为0.2 ~ 0.6 mL/h，外流速为1.8 mL/h，制备了0.85 ~ 2.17 mg/g的fitcBSA纳米纤维膜。流速的变化也导致纤维尺寸从约270 nm增加到380 nm。随后，通过激光共聚焦扫描显微镜、透射电子显微镜（TEM）和x射线光电子能谱（XPS）分析对fitcBSA/PEG在PCL中的封装进行了表征。体外释放研究评估了核-鞘结构复合纳米纤维PCL-r-fitcBSA/PEG的缓释潜力。作为阴性对照，采用静电纺丝法制备了PCL/fitcBSA/PEG共混复合纳米纤维。研究发现，与PCL/fitcBSA/PEG纳米纤维相比，芯鞘纳米纤维PCL-r-fitcBSA/PEG明显减轻了蛋白质负载的初始爆发释放，并具有更好的可持续性。本研究将为进一步设计和优化加工条件提供基础，以控制芯鞘复合纳米纤维的纳米结构，最终实现生物活性蛋白（如生长因子）的释放动力学，为实际组织工程应用提供依据。
As an aim toward developing biologically mimetic and functional nanofiber-based tissue engineering scaffolds. we demonstrated the encapsulation of a model protein, fluorescein isothiocyanate-conjugated bovine serum albumin (fitcBSA), along with a water-soluble polymer, poly(ethylene glycol) (PEG), within the biodegradable poly(epsilon-caprolactone) (PCL) nanofibers using a coaxial electrospinning technique. By variation of the inner flow rates from 0.2 to 0.6 mL/h with a constant outer flow rate of 1.8 mL/h, fitcBSA loadings of 0.85-2.17 mg/g of nanofibrous membranes were prepared. Variation of flow rates also resulted in increases of fiber sizes from ca. 270 nm to 380 nm. The encapsulation of fitcBSA/PEG within PCL was subsequently characterized by laser confocal scanning microscopy, transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) analysis. In vitro release Studies were conducted to evaluate sustained release potential of the core-sheath-structured composite nanofiber PCL-r-fitcBSA/PEG. As a negative control, composite nanofiber PCL/fitcBSA/PEG blend was prepared from a normal electrospinning method. It was found that core-sheath nanofibers PCL-r-fitcBSA/PEG pronouncedly alleviated the initial burst release for higher protein loading and gave better sustainability compared to that of PCL/fitcBSA/PEG nanofibers. The present study would provide a basis for further design and optimization of processing conditions to control the nanostructure of core-sheath composite nanofibers and ultimately achieve desired release kinetics of bioactive proteins (e.g., growth factors) for practical tissue engineering applications.