Development and mapping of a public reference set of SSR markers in Lolium perenne L.

Development and mapping of a public reference set of SSR markers in Lolium perenne L.
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DOI:
10.1111/j.1471-8286.2005.01043.x
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发表时间:
2005-12-01
期刊:
MOLECULAR ECOLOGY NOTES
影响因子:
--
通讯作者:
Barre, P
Barre, P
中科院分区:
其他
文献类型:
--
作者:
Jensen, LB;Muylle, H;Barre, P

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我们报告了黑麦草 76 个简单序列重复 (SSR) 标记的表征和作图。这些标记是公开可用的,或者从富含 SSR 基序的基因组文库或多年生黑麦草表达序列标签 (EST) 克隆中获得。使用四个多年生黑麦草作图群体来绘制 SSR 标记。提供了包含 65 个 SSR 标记的四个作图群体的共有连锁图,以及引物信息和表明 SSR 标记在不同群体中有用性的质量评分。 SSR 标记鉴定了所有七个多年生黑麦草连锁群。
We report on the characterization and mapping of 76 simple sequence repeat (SSR) markers for Lolium perenne. These markers are publicly available or obtained either from genomic libraries enriched for SSR motifs or L. perenne expressed sequence tag (EST) clones. Four L. perenne mapping populations were used to map the SSR markers. A consensus linkage map of the four mapping populations containing 65 of the SSR markers is presented, together with primer information and a quality score indicating the usefulness of the SSR marker in different populations. The SSR markers identified all seven L. perenne linkage groups.