Involvement of Cajal-Retzius neurons in spontaneous correlated activity of embryonic and postnatal layer 1 from wild-type and reeler mice
Involvement of Cajal-Retzius neurons in spontaneous correlated activity of embryonic and postnatal layer 1 from wild-type and reeler mice
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DOI：
10.1523/jneurosci.19-24-10856.1999
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发表时间：
1999-12-15
影响因子：
5.3
通讯作者：
Yuste, R
中科院分区：
文献类型：
--
作者：
Aguiló, A;Schwartz, TH;Yuste, R
Cajal-Retzius（CR）细胞是发育中的皮质层1中的短暂神经元群体，其分泌皮质层压所必需的蛋白质reelin。结合钙成像的皮质半球和交叉相关分析，我们以前发现自发相关活动之间的非CR神经元在出生后的大鼠层1。这种相关活性被GABA能和谷氨酸能拮抗剂阻断，我们推测它是由CR细胞控制的。我们现在调查的相关活动的胚胎和出生后的第1层在野生型和reeler小鼠，突变体的生产reelin。我们发现，小鼠第1层也维持模式化的自发活动，CR细胞参与相关网络。这些网络存在于胚胎边缘区，并被GABA能和谷氨酸能拮抗剂阻断。令人惊讶的是，在reeler小鼠中的网络活动显示出与野生型小鼠相似的特征和药理学特征，尽管检测到了小的差异。我们的研究结果表明，胚胎边缘区有相关的自发活动，可以作为支架的发展皮质内连接。我们的数据还表明，reelin没有在第1层的特定突触电路的发展产生重大影响。
Cajal-Retzius (CR) cells are a transient population of neurons in developing cortical layer 1 that secrete reelin, a protein necessary for cortical lamination. Combining calcium imaging of cortical hemispheres and cross-correlation analysis, we previously found spontaneous correlated activity among non-CR neurons in postnatal rat layer 1. This correlated activity was blocked by GABAergic and glutamatergic antagonists, and we postulated that it was controlled by CR cells. We now investigate the correlated activity of embryonic and postnatal layer 1 in wild-type and reeler mice, mutant in the production of reelin. We find that mouse layer 1 also sustains patterned spontaneous activity and that CR cells participate in correlated networks. These networks are present in embryonic marginal zone and are blocked by GABAergic and glutamatergic antagonists. Surprisingly, network activity in reeler mice displays similar characteristics and pharmacological profile as in wild-type mice, although small differences are detected. Our results demonstrate that the embryonic marginal zone has correlated spontaneous activity that could serve as the scaffold for the development of intracortical connections. Our data also suggest that reelin does not have a major impact in the development of specific synaptic circuits in layer 1.