DNA methylation changes induced by long and short photoperiods in Nasonia.
DNA methylation changes induced by long and short photoperiods in Nasonia.
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鼻孔长和短光周期引起的DNA甲基化变化。
DOI：
10.1101/gr.196204.115
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发表时间：
2016-02
期刊：
影响因子：
7
通讯作者：
Tauber E
中科院分区：
文献类型：
--
作者：
Pegoraro M;Bafna A;Davies NJ;Shuker DM;Tauber E
许多生物监测白昼长度的年度变化，并利用这些信息来确定其季节性反应的时间。然而，光周期计时的分子机制在很大程度上是未知的。vitripennis黄蜂是一种新兴的模式生物，表现出强烈的光周期反应：雌性经历的短暂秋季导致其后代发育停滞（滞育），使幼虫能够在冬季存活。雌性纳索尼亚龙如何控制其后代的发育轨迹尚不清楚。在这里，我们利用了最近的发现，DNA甲基化在纳索尼亚普遍存在，并测试了它在光周期症中的作用。我们使用亚硫酸氢盐还原测序（RRBS）分析了不同光周期下成年雌性黄蜂的DNA甲基化，并在单碱基水平上发现了实质性的差异甲基化。我们还表明，敲低DNA甲基转移酶1a （Dnmt1a）、Dnmt3或从药理学上阻断DNA甲基化，在很大程度上破坏了黄蜂的光周期滞育反应。据我们所知，这是DNA甲基化在昆虫光周期计时中的作用的第一个例子。
Many organisms monitor the annual change in day length and use this information for the timing of their seasonal response. However, the molecular mechanisms underlying photoperiodic timing are largely unknown. The wasp Nasonia vitripennis is an emerging model organism that exhibits a strong photoperiodic response: Short autumnal days experienced by females lead to the induction of developmental arrest (diapause) in their progeny, allowing winter survival of the larvae. How female Nasonia control the developmental trajectory of their offspring is unclear. Here, we took advantage of the recent discovery that DNA methylation is pervasive in Nasonia and tested its role in photoperiodism. We used reduced representation bisulfite sequencing (RRBS) to profile DNA methylation in adult female wasps subjected to different photoperiods and identified substantial differential methylation at the single base level. We also show that knocking down DNA methyltransferase 1a (Dnmt1a), Dnmt3, or blocking DNA methylation pharmacologically, largely disrupts the photoperiodic diapause response of the wasps. To our knowledge, this is the first example for a role of DNA methylation in insect photoperiodic timing.