Expression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patient
Expression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patient
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DOI：
10.3892/ol.2012.711
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发表时间：
2012-08-01
期刊：
影响因子：
2.9
通讯作者：
Sriuranpong, Virote
中科院分区：
文献类型：
--
作者：
Chanvorachote, Pithi;Luanpitpong, Sudjit;Sriuranpong, Virote
对肺癌生物学和肿瘤标志物的了解有助于临床医生管理这种疾病。癌症相关抗原（CA）125在肺癌研究中受到越来越多的关注，并可能有益于此类癌症的治疗和随访。在泰国肺癌患者中，关于癌细胞生物学种族差异的知识基本上是不存在的。我们从泰国患者的胸腔积液中产生肺癌细胞，并将其命名为PI细胞。评估PI细胞的生长速率、对化疗的反应和肿瘤标志物的存在，特别是CA 125表达。免疫荧光结果显示PI细胞表现出与已建立的H460肺癌细胞相当的CA 125强表达水平。此外，分析PI细胞的其他标志物的表达。结果显示，H460细胞表现出来自细胞角蛋白-19片段（CYFRA 21-1）和鳞状细胞癌抗原（SCCA）的强免疫荧光信号，而PI仅呈现CYFRA 21-1信号。我们还发现了相对顺铂耐药的证据相比，建立肺癌细胞的敏感性水平在PI。因此，本研究中描述的结果和方法可能有助于肺癌诊断和治疗方法的发展，特别是促进对种族差异的理解。
Advances in understanding lung cancer biology and tumor markers aid clinicians in managing the disease. Cancer-associated antigen (CA)125 has garnered increasing attention in lung cancer research and may benefit the treatment and follow-up of this type of cancer. In Thai lung cancer patients, knowledge regarding ethnic differences in cancer cell biology is largely absent. We generated lung cancer cells from the pleural effusion fluids of a Thai patient and designated these as PI cells. PI cells were assessed for growth rate, response to chemotherapy, and the presence of tumor markers, in particular CA 125 expression. Results of immunofluorescence indicated that PI cells exhibited strong expression levels of CA 125, comparable to that of established H460 lung cancer cells. Furthermore, PI cells were analyzed for the expression of additional markers. Results revealed that H460 cells exhibited strong immunofluorescent signals from cytokeratin-19 fragments (CYFRA 21-1) and squamous cell carcinoma antigen (SCCA) while PI presented only CYFRA 21-1 signals. We also found evidence of relative cisplatin resistance in PI compared to the susceptibility level of established lung cancer cells. Thus, the results and methodology described in this study may aid the development of lung cancer diagnostic and therapeutic approaches and, in particular, advance understanding of ethnic differences.