Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production
Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production
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DOI：
10.1002/bit.20550
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发表时间：
2005-09-30
影响因子：
3.8
通讯作者：
Park, TH
中科院分区：
文献类型：
--
作者：
Choi, SS;Rhee, WJ;Park, TH
为了生产促红细胞生成素（EPO），首先在含有FBS的培养基（生长培养基）中培养中国仓鼠卵巢（CHO）细胞，然后在含有丁酸钠的无血清培养基（生产培养基）中培养。丁酸钠增加重组蛋白的产生，但也诱导细胞凋亡，这降低了细胞活力和生产力。在以前的研究中，我们发现家蚕血淋巴（SH），一种昆虫血清，抑制昆虫和哺乳动物细胞的凋亡。为了克服丁酸钠诱导的细胞凋亡，我们在生长培养基中加入SH。SH预处理抑制了丁酸钠诱导的CHO细胞凋亡，从而增加了它们的寿命和产生EPO的能力。结果，EPO的体积生产率提高了五倍。发现SH抑制细胞色素c从线粒体释放到胞质溶胶中，并阻止caspase-3的活化和其他随后的caspase反应。(c)2005 Wiley Periodicals，Inc.
To produce erythropoietin (EPO), Chinese hamster ovary (CHO) cells were first cultured in a medium containing FBS (growth medium) and then in a serumfree medium containing sodium butyrate (production medium). Sodium butyrate increases recombinant protein production, but also induces apoptosis, which reduces cell viability and productivity. In a previous study, we found that silkworm hemolymph (SH), an insect serum, inhibits the apoptosis of insect and mammalian cells. To overcome sodium butyrate-induced apoptosis, we added SH to growth medium. This pretreatment with SH inhibited the sodium butyrate-induced apoptosis of CHO cells and consequently increased their longevity and their ability to produce EPO. As a result, the volumetric productivity of EPO was increased five-fold. SH was found to inhibit cytochrome c release from mitochondria into the cytosol, and prevented the activation of caspase-3 and other subsequent caspase reactions. (c) 2005 Wiley Periodicals, Inc.