STRUCTURAL CONSERVATION IN THE CHEY SUPERFAMILY
STRUCTURAL CONSERVATION IN THE CHEY SUPERFAMILY
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DOI：
10.1021/bi00095a001
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发表时间：
1993-11-09
期刊：
影响因子：
2.9
通讯作者：
VOLZ, K
中科院分区：
文献类型：
--
作者：
VOLZ, K
细菌适应性地对在其不可预测的环境中遇到的各种各样的刺激做出反应。它们的免疫应答使宿主生物体中感染状态的建立和消除以及在外部环境中的存活成为可能。细菌适应是通过协调激活许多不同的感觉受体、信号处理蛋白和一系列旨在执行适当反应的分子机制来实现的。许多这些行为反应的关键控制点是基于原型双组分范式的信号处理途径。这种系统的主要传感元件是一种自磷酸化蛋白激酶，而第二个元件--反应调节因子--在接收到来自第一个元件的磷酰基后被激活。“双组分”系统已经在多达20种不同的细菌信号转导途径中被鉴定，涉及30多个原核生物属。各个系统的操作根据其特定任务而变化很大，但对于响应调节，它们可能利用Mg 2+依赖性蛋白磷酸化的相同激活机制。在这么多不同的通路中保留一个共同的信号机制背后的分子逻辑可能在于调节串扰的能力，从而实现一个复杂的，协调一致的反应措施。虽然反应调节因子在其结构域组织和全长一级序列中表现出很大的多样性，但它们在其调节域中都有相似性。这个超家族的一个成员是CheY，细菌趋化系统的单域调节剂。CheY是唯一一个详细的三维结构已知的响应调节器。在这份报告中，CheY分子是作为所有细菌双组分系统的调节结构域的结构模型。分析了79个调节结构域序列的多重比对。的相关性
Bacteria adaptively respond to a wide varietyof stimuli encountered in their unpredictableenvironment. Their be-havioral responses enable establishment and maintainance of infectious states in host organisms, as well as survival in the external milieu. Bacterial adaptation is accomplished through the coordinated activation of many different sensory receptors, signal processing proteins, and an array of molecular ma-chinery designed to execute the appropriate reactions. Key control points for many of these behavioral responses are signal processing pathways based on an archetypal two-component paradigm. The primary sensing component of such a system is an autophosphorylating protein kinase, while thesecond component—the response regulator—becomesactivated after receiving the phosphoryl group from the first.“Two-component” systems have been identified in as many as 20 distinct bacterial signal transductionpathways, ranging over30 prokaryotic genera. Operations of the individual systems vary widely according to their specific tasks, but for response regulation, it is likely that they allutilize the same activation mechanism of Mg2+-dependent protein phosphorylation. The molecular logic behind preservation of a common signaling mechanism in so many different pathways may lie in the ability to modulate crosstalk, thereby achieving a complex, concerted measure of response.Although response regulators exhibit great diversity in their domain organization and full-length primary sequences, they all share similarity in their regulatorydomain. One member of this superfamily is CheY, the single domain regulator of the bacterial chemotaxis system. CheY is the only response regulator for which a detailed three-dimensional structure is known. In thisreport, the CheY molecule is presented as the structural model for the regulatory domains of all bacterial two-component systems. A multiple alignment of 79 regu-latory domain sequences is analyzed. The correlation of the