Antibody probing of western blots which have been stained with india ink.
Antibody probing of western blots which have been stained with india ink.
复制标题
对用印度墨水染色的蛋白质印迹进行抗体探测。
DOI：
10.1016/0003-2697(86)90259-9
复制
发表时间：
1986
影响因子：
2.9
通讯作者：
Glenney,J
中科院分区：
文献类型：
--
作者：
Glenney,J
印度墨水可用于染色与硝酸纤维素结合的蛋白质。随后，可以用抗体和125 I-蛋白A鉴定特定蛋白质。在大多数情况下，墨汁不会显著抑制抗体检测，在抗体孵育步骤中墨汁也不会被洗掉。使用这种方法，可以用相同的复制品直接比较抗体反应蛋白和总蛋白。
India ink can be used to stain proteins bound to nitrocellulose. Subsequently, specific proteins can be identified with antibodies and125I-protein A. In most cases, india ink did not significantly inhibit detection with antibody, nor was the ink washed off during the antibody incubation steps. Using this method, a direct comparison of antibody-reactive protein and total protein can be made with the same replica.