CaMADS, a MADS-box transcription factor from pepper, plays an important role in the response to cold, salt, and osmotic stress
CaMADS, a MADS-box transcription factor from pepper, plays an important role in the response to cold, salt, and osmotic stress
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CaMADS 是一种来自辣椒的 MADS-box 转录因子，在对寒冷、盐和渗透胁迫的反应中发挥着重要作用
DOI：
10.1016/j.plantsci.2018.11.020
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发表时间：
2019-03-01
期刊：
影响因子：
5.2
通讯作者：
Guo, Weili
中科院分区：
文献类型：
--
作者：
Chen, Rugang;Ma, Jihui;Guo, Weili
MADS-box家族转录因子在植物的生长发育中起着重要的作用，一些MADS-box基因也被发现参与了植物的逆境应答。目前，关于辣椒中与胁迫相关的MADS盒基因的信息很少。我们从辣椒中克隆了一个MADS盒转录因子基因，命名为CaMADS。CaMADS表达受低温和高温、盐和渗透胁迫诱导，也受脱落酸（阿坝）、水杨酸（SA）、甲基茉莉酸（MeJA）和CaCl 2诱导。为了了解CaMADS在非生物胁迫反应中的功能，我们产生了辣椒植物，其中CaMADS表达使用VIGS（病毒诱导的基因沉默）下调，也过表达CaMADS的转基因拟南芥植物。我们发现，CaMADS下调的幼苗更严重的伤害比WT后冷，NaCl，甘露醇处理，并表现出增加电解质渗漏，丙二醛（MDA）水平，和较低的叶绿素含量。过表达CaMADS的拟南芥植株比野生型更能耐受这些胁迫，并且在冷处理后表现出显著高的存活率和较低的H2 O2和超氧自由基含量。CaMADS过表达植株在NaCl和甘露醇处理后具有较高的发芽率和绿色子叶百分比。CaMADS过表达植物的根长和鲜重也分别比WT植物显著更长和更高。两者合计，我们的研究结果表明，CaMADS功能作为一个积极的压力响应转录因子的冷，盐，渗透胁迫信号通路。
MADS-box family transcription factors play essential roles in the growth and development of plants, and some MADS-box genes have also been found to participate in the stress-response. At present, little information regarding stress-related MADS-box genes is available in pepper. We isolated a MADS-box transcription factor gene from Capsicum annum, which we named CaMADS. CaMADS expression is induced by low and high temperature, salt, and osmotic stress, and also by abscisic acid (ABA), salicylic acid (SA), methyl-jasmonic acid (MeJA), and CaCl2. To understand the function of CaMADS in the abiotic stress response, we generated pepper plants in which CaMADS expression was down-regulated using VIGS (Virus-induced Gene Silencing), and also transgenic Arabidopsis plants overexpressing CaMADS. We found that CaMADS-down-regulated seedlings were more seriously injured than WT after cold, NaCl, and mannitol treatment, and showed increased electrolyte leakage, malondialdehyde (MDA) levels, and lower chlorophyll content. CaMADS-overexpressing Arabidopsis plants were more tolerant to these stresses than WT, and showed significantly high survival rates and lower H2O2 and super oxide radical contents after cold treatment. CaMADS-overexpressing plants had higher germination rates and percentages of green cotyledons following NaCl and mannitol treatment. Root lengths and fresh weight in CaMADS-overexpressing plants were also significantly longer and higher, respectively, than in WT plants. Taken together, our results suggest that CaMADS functions as a positive stress-responsive transcription factor in the cold, salt, and osmotic stress signaling pathways.