Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A
Effect of Polycyclic Aromatic Hydrocarbons on Development of the Ascidian Ciona intestinalis Type A
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DOI：
10.3390/ijerph17041340
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发表时间：
2020-02-01
影响因子：
--
通讯作者：
Wada, Shuichi
中科院分区：
文献类型：
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作者：
Sekiguchi, Toshio;Akitaya, Hiroshi;Wada, Shuichi
多环芳烃（PAHs）是一种对海洋无脊椎动物有危害的污染物，但其作用的分子机制尚不清楚。研究了多环芳烃对海鞘Ciona robusta A型的影响。首先，多环芳烃对玻璃海鞘早期发育的影响进行了评估。多环芳烃，如二苯并噻吩，芴，菲导致异常幼虫的形成。多环芳烃处理游泳幼虫会导致尾部退化形式的畸形。此外，我们观察了Cionaaryl hydrocarbon receptor（Ci-AhR）mRNA在游泳幼虫、中轴旋转和早期幼鱼阶段的表达。PAH作用与AhR mRNA表达之间的时间相关性表明，Ci-AhR可能与PAH代谢有关。最后，我们分析了Ci-AhR mRNA定位在玻璃海鞘青少年。Ci-AhR mRNA定位于消化道，背结节，神经节，鳃囊的乳头，这表明Ci-AhR是一个候选人的环境污染物传感器，并执行神经功能。我们的研究结果提供了基础知识的Ci-AhR和PAH活性的海洋无脊椎动物的生物学功能。
Polycyclic aromatic hydrocarbons (PAHs) are pollutants that exert harmful effects on marine invertebrates; however, the molecular mechanism underlying PAH action remains unclear. We investigated the effect of PAHs on the ascidian Ciona intestinalis type A (Ciona robusta). First, the influence of PAHs on early Ciona development was evaluated. PAHs such as dibenzothiophene, fluorene, and phenanthrene resulted in formation of abnormal larvae. PAH treatment of swimming larva induced malformation in the form of tail regression. Additionally, we observed the Cionaaryl hydrocarbon receptor (Ci-AhR) mRNA expression in swimming larva, mid body axis rotation, and early juvenile stages. The time correlation between PAH action and AhR mRNA expression suggested that Ci-AhR could be associated with PAH metabolism. Lastly, we analyzed Ci-AhR mRNA localization in Ciona juveniles. Ci-AhR mRNA was localized in the digestive tract, dorsal tubercle, ganglion, and papillae of the branchial sac, suggesting that Ci-AhR is a candidate for an environmental pollutant sensor and performs a neural function. Our results provide basic knowledge on the biological function of Ci-AhR and PAH activity in marine invertebrates.