Analytical procedures and methods validation for oxalate content estimation.
Analytical procedures and methods validation for oxalate content estimation.
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DOI：
10.33263/briac95.305310
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发表时间：
2019-10-15
影响因子：
--
通讯作者：
Tavasoli S
中科院分区：
文献类型：
--
作者：
Karamad D;Khosravi-Darani K;Hosseini H;Tavasoli S
尿草酸盐增加被认为是草酸钙肾结石形成的主要危险因素。肠道微生物群可以降低结石形成的风险。无论如何，任何关于监测草酸盐含量（体外和体内）的研究的第一步都是确定其浓度，而文献中报道的草酸盐含量测定方法不同。在这项研究中，主要报道的方法，包括滴定与两个滴定器（高锰酸钾，和NaOH）以及酶法（草酸盐测定试剂盒），并比较在接种和未接种的培养基中的草酸盐的测量。
Increased urinary oxalate is considered a major risk factor in the formation of calcium oxalate kidney stones. Gut microbiota may reduce the risk of stone formation. Anyway, the first step for any research about monitoring of oxalate content (both in vitro and in vivo) is a determination of its concentration, while there are different methods reported in the literature for oxalate content determination. In this research, the main reported methods including titration with two titrators (potassium permanganate, and NaOH) as well as enzymatic method (oxalate assay kit) are presented and compared for the measurement of oxalate in both inoculated and non-inoculated media.