Biosensors based on gold nanostructures
Biosensors based on gold nanostructures
复制标题
DOI：
10.1590/s0103-50532011000100002
复制
发表时间：
2011
影响因子：
1.4
通讯作者：
M. Vidotti;R. F. Carvalhal;R. K. Mendes;D. C. M. Ferreira;L. Kubota
中科院分区：
文献类型：
--
作者：
M. Vidotti;R. F. Carvalhal;R. K. Mendes;D. C. M. Ferreira;L. Kubota
本文综述了生物传感器技术的最新进展，通过组装生物分子与金纳米粒子在分析装置中实现。本综述根据生物传感器开发中使用的生物分子分为几个部分：（i）免疫化合物;（ii）DNA/RNA和功能性DNA/RNA;（iii）酶和血红素蛋白。为了便于理解，根据转导模式将每个部分细分。基于纳米金的免疫传感器在食品、环境、制药、化学和临床诊断等领域有着广泛的应用。纳米颗粒被用来改善分析信号或免疫化合物的固定。在另一部分中，系统地报道了基于DNA/RNA生物分子的生物传感器，其采用金纳米结构作为标记物和与金纳米结构相关联的无标记功能DNA/RNA生物传感器作为传感器，分别用于快速鉴定病原体、环境感兴趣的物种和临床诊断。在修饰电极中包含金纳米颗粒本身增强了换能器和生物分子之间的电子转移，从而在使用氧化还原酶和血红素蛋白时改善了生物分析装置。生物传感器的检测和定量葡萄糖和过氧化氢进行了讨论。
The present review discusses the latest advances in biosensor technology achieved by the assembly of biomolecules associated with gold nanoparticles in analytical devices. This review is divided in sections according to the biomolecule employed in the biosensor development: (i) immunocompounds; (ii) DNA/RNA and functional DNA/RNA; and (iii) enzymes and Heme proteins. In order to facilitate the comprehension each section was subdivided according to the transduction mode. Gold nanoparticles based immunosensors have a wide range of applications in food, environmental, pharmaceutical, chemistry and clinical diagnostics. The nanoparticles were employed to improve whether the analytical signal or the immunocompounds immobilization. In another section, biosensors based on DNA/RNA biomolecules employing gold nanostructures as labels and label-free funtional DNA/RNA biosensors associated to gold nanostructures as tranducers were systematically reported for rapid identification of pathogens, species of environmental interest and clinical diagnostics, respectively. The inclusion of gold nanoparticles in modified electrodes itself enhances the electron transfer between the transducer and biomolecules leading to improved bioanalytical devices when redox enzymes and heme proteins are used. Biosensors for the detection and quantification of glucose and hydrogen peroxide are discussed as well.