A simple method for squeezing juice from rice stems and its use in the high-throughput analysis of sugar content in rice stems.
A simple method for squeezing juice from rice stems and its use in the high-throughput analysis of sugar content in rice stems.
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一种简单的稻秆榨汁方法及其在稻秆糖含量高通量分析中的应用。
DOI：
10.1080/1343943x.2015.1128099
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Aoki N
中科院分区：
文献类型：
--
作者：
Okamura M;Hashida;Y;Hirose T;Ohsugi R;Aoki N
水稻（Oryza sativaL.）茎中糖含量是秸秆青贮或全株青贮水稻的重要农艺性状。然而，水稻茎中糖积累的机制尚不清楚，主要是由于测量糖含量的方法耗时。在这里，我们建立了一种简单的从水稻植株中榨取茎汁的方法，类似于在甘蔗或高粱的育种选择中使用的方法。茎汁的白度值与茎汁中的可溶性糖含量呈显著相关，且与茎组织中的可溶性糖含量呈显著相关。这表明茎汁的白利度值可以用来估计水稻茎中的糖含量。这种简便的估算方法将为突变体筛选、QTL分析和育种选择的高通量分析水稻茎中糖含量提供有用的工具。
Sugar content in rice (Oryza sativaL.) stem is an agronomically important trait for rice used in straw silage or whole-crop silage. However, the mechanisms underlying sugar accumulation in rice stems remain unclear, mainly due to the time-consuming method for measuring sugar content. Here, we established a simple method for squeezing stem juice from rice plants, similar to that used during breeding selection in sugarcane or sorghum. The Brix value of the stem juice, which can easily be measured using a portable refractometer, significantly correlated with the soluble sugar contents in the stem juice and tended to correlate with those in stem tissues. This indicates that the Brix value of the stem juice can be used for estimating the sugar content in rice stems. This simple estimation method will be a useful tool for high-throughput analysis of sugar content in rice stems during mutant screening, QTL analysis, and breeding selection.