Recombinant murine granulocyte macrophage colony-stimulating factor has megakaryocyte colony-stimulating activity and augments megakaryocyte colony stimulation by interleukin 3.
Recombinant murine granulocyte macrophage colony-stimulating factor has megakaryocyte colony-stimulating activity and augments megakaryocyte colony stimulation by interleukin 3.
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重组鼠粒细胞巨噬细胞集落刺激因子具有巨核细胞集落刺激活性，可增强白细胞介素 3 对巨核细胞集落的刺激。
DOI：
10.1172/jci113002
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Quesenberry,PJ
中科院分区：
文献类型：
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作者：
Robinson,BE;McGrath,HE;Quesenberry,PJ
重组小鼠粒细胞巨噬细胞集落刺激因子（rGM-CSF）在大肠杆菌中产生并纯化至均质性。GM-CSF已被确定为粒细胞和巨噬细胞集落形成的体外调节剂。我们已经确定rGM-CSF也具有作为巨核细胞集落刺激因子的内在活性，并且rGM-CSF增强了白细胞介素3 （IL-3）对巨核细胞集落形成的作用。rGM-CSF诱导巨核细胞集落的剂量-反应曲线显示，在9 ng/ml时，巨核细胞刺激达到平台。当IL-3（用于巨核细胞集落诱导的平台剂量）以0-22 ng/ml的剂量范围添加到rGM-CSF中时，所产生的巨核细胞集落刺激接近单独产生的任何一种因子的刺激水平的总和。这些结果证实了GM-CSF是一种多系生长因子，具有明确的巨核细胞集落刺激活性，并表明GM-CSF和IL-3在巨核细胞生成的调节中都很重要。
Recombinant murine granulocyte macrophage colony-stimulating factor (rGM-CSF) has been produced in Escherichia coli and purified to homogeneity. GM-CSF has an established role as an in vitro regulator of granulocyte and macrophage colony formation. We have determined that rGM-CSF also has intrinsic activity as a megakaryocyte colony-stimulating factor and that rGM-CSF augments the effect of interleukin 3 (IL-3) on megakaryocyte colony formation. The dose-response curve for megakaryocyte colony induction with rGM-CSF showed plateau megakaryocyte stimulation at 9 ng/ml. When IL-3 (at a plateau dose for megakaryocyte colony induction) was added to rGM-CSF over a 0-22-ng/ml dose range, the resultant megakaryocyte colony stimulation approximated the sum of the levels of stimulation produced by either factor alone. These results establish GM-CSF as a multilineage growth factor with definite megakaryocyte colony-stimulating activity and indicate that both GM-CSF and IL-3 are important in the regulation of megakaryocytopoiesis.