Signal Destruction Tunes the Zone of Activation in Spatially Distributed Signaling Networks
Signal Destruction Tunes the Zone of Activation in Spatially Distributed Signaling Networks
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DOI：
10.1016/j.bpj.2017.01.010
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发表时间：
2017-03-14
影响因子：
3.4
通讯作者：
Boedicker, James Q.
中科院分区：
文献类型：
--
作者：
Silva, Kalinga Pavan;Chellamuthu, Prithiviraj;Boedicker, James Q.
不同的微生物群落通过信号交换协调群体行为，例如革兰氏阴性菌交换酰基高丝氨酸内酯（AHLs）。细胞通信容易受到邻近微生物的干扰。干扰的一种机制是通过产生切割信号分子的酶来破坏信号。在这里，我们研究了一种这样的干扰酶AiiA调节细菌空间分布系统中信号传播的能力。我们已经开发了一种实验测定来测量信号转导，并实施信号动力学的理论模型来预测系统如何响应干扰。我们表明，滴定的干扰应变到信号网络调谐激活的空间范围超过厘米的长度尺度，量化的信号网络的鲁棒性信号破坏和展示的能力，程序系统级响应的空间异质性细胞网络。
Diverse microbial communities coordinate group behaviors through signal exchange, such as the exchange of acyl-homoserine lactones (AHLs) by Gram-negative bacteria. Cellular communication is prone to interference by neighboring microbes. One mechanism of interference is signal destruction through the production of an enzyme that cleaves the signaling molecule. Here we examine the ability of one such interference enzyme, AiiA, to modulate signal propagation in a spatially distributed system of bacteria. We have developed an experimental assay to measure signal transduction and implement a theoretical model of signaling dynamics to predict how the system responds to interference. We show that titration of an interfering strain into a signaling network tunes the spatial range of activation over the centimeter length scale, quantifying the robustness of the signaling network to signal destruction and demonstrating the ability to program systems-level responses of spatially heterogeneous cellular networks.