Recombinant human bone morphogenetic protein-7 induces healing in a canine long-bone segmental defect model.
Recombinant human bone morphogenetic protein-7 induces healing in a canine long-bone segmental defect model.
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DOI：
10.1097/00003086-199404000-00046
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发表时间：
1994-04
影响因子：
4.2
通讯作者：
Stephen D. Cook;G. C. Baffes;M. M. Wolfe-M.;T. K. Sampath;D. Rueger
中科院分区：
文献类型：
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作者：
Stephen D. Cook;G. C. Baffes;M. M. Wolfe-M.;T. K. Sampath;D. Rueger
在成年雄性狗中使用尺骨节段性缺损模型来检查重组人骨形态发生蛋白-7（重组人成骨蛋白-1 [rhOP-1]; Creative Biomolecules，Hopkinton，马萨诸塞州）对新骨诱导和愈合的作用，并测试愈合的2.5-cm节段性骨缺损的机械强度。rhOP-1复合物由500 mg脱矿物质、胍提取、不溶性牛骨基质（胶原蛋白载体）的载体组成，用rhOP-1重构。6只动物单侧接受1200 μ g rhOP-1，并在12周时处死，进行扭转载荷失效测试，使用对侧作为对照。另外两只动物双侧接受不同量的rhOP-1，并进行组织学研究。所有接受rhOP-1治疗的缺损部位在8周时通过放射学检查完全桥接。不含rhOP-1的对照复合物在任何时间都不能诱导新骨形成。在组织学上，在16周时检查的rhOP-1处理部位形成了新的皮质骨和松质骨，在重建的髓管中具有正常出现的骨髓元素。植入rhOP-1的尺骨的扭转强度平均为对照的72%（范围，30-99%）。失效角变形平均为对照组的92%（范围：39-122%）。失效能量吸收平均为对照的67%（范围，27-111%）。本研究证明了rhOP-1在犬模型中愈合节段性骨骨膜缺损的功效。
An ulnar segmental defect model was used in adult male dogs to examine the effect of recombinant human bone morphogenetic protein-7 (recombinant human Osteogenic Protein-1 [rhOP-1]; Creative Biomolecules, Hopkinton, Massachusetts) on new bone induction and healing, and to test the mechanical strength of healed 2.5-cm segmental bone defects. The rhOP-1 composites consisted of a carrier of 500 mg of demineralized, guanidine-extracted, insoluble bovine bone matrix (collagen carrier), reconstituted with rhOP-1. Six animals received 1200 micrograms rhOP-1 unilaterally and were killed at 12 weeks for torsional load-to-failure testing using the contralateral side as a control. Two further animals received varying amounts of rhOP-1 bilaterally and were studied histologically. All defect sites receiving rhOP-1 were completely bridged radiographically by eight weeks. A control composite, containing no rhOP-1, failed to induce new bone formation at any time. Histologically, rhOP-1-treated sites examined at 16 weeks had formation of new cortical and cancellous bone, with normal appearing marrow elements in the reconstituted medullary canal. The torsional strength of the rhOP-1-implanted ulnae averaged 72% of control (range, 30-99%). The angular deformation to failure averaged 92% of control (range, 39-122%). The energy absorption to failure averaged 67% of control (range, 27-111%). This study demonstrates the efficacy of rhOP-1 in healing segmental osteoperiosteal defects in a canine model.