IMMUNOCHEMICAL CHARACTERIZATION OF 2 SURFACE POLYSACCHARIDES OF BACTEROIDES-FRAGILIS
IMMUNOCHEMICAL CHARACTERIZATION OF 2 SURFACE POLYSACCHARIDES OF BACTEROIDES-FRAGILIS
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DOI：
10.1128/iai.59.6.2075-2082.1991
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发表时间：
1991-06-01
影响因子：
3.1
通讯作者：
KASPER, DL
中科院分区：
文献类型：
--
作者：
PANTOSTI, A;TZIANABOS, AO;KASPER, DL
对脆弱拟杆菌NCTC 9343的荚膜多糖进行免疫化学分析，发现了一种由两种不同多糖组成的新结构。 从发酵生长的生物体的纯化的表面多糖的提取物的免疫电泳显示出不同的阳极迁移率的复杂的沉淀素配置文件。 多糖提取物的DEAE-Sephacel阴离子交换色谱未能分离大部分这种聚集体。 通过非常温和的酸处理完成该复合物的解聚;通过DEAE-Sephacel阴离子交换色谱实现纯化。 多糖A在pH 7.3时带中性电荷，在pH 8.6时带净负电荷，平均M（r）= 110，000;化学分析表明它含有半乳糖、半乳糖胺和一种未鉴定的氨基糖。 多糖B用增加的盐浓度从阴离子交换柱洗脱;其具有净负电荷和平均M（r）= 200，000，并且含有岩藻糖、半乳糖、喹诺糖胺、半乳糖醛酸和葡糖胺。 这两种多糖均不含可检测的3-脱氧-D-甘露-辛糖醛酸，并且两者都被识别为不同的抗原，基于它们与单克隆抗体CE 3和F10的反应性，所述单克隆抗体CE 3和F10在酸处理之前与复合物反应。 这些数据表明，B. fragilis NCTC 9343包含两种离散的、表面暴露的多糖，其具有不同于该生物体的脂多糖的不同物理化学性质。 两种表面多糖的发现还没有被描述为其他对人类致病的细菌。
Immunochemical analysis of the capsular polysaccharide from Bacteroides fragilis NCTC 9343 revealed a novel structure composed of two distinct polysaccharides. Immunoelectrophoresis of an extract of purified surface polysaccharide from fermenter-grown organisms showed a complex precipitin profile with varying anodal mobility. DEAE-Sephacel anion-exchange chromatography of the polysaccharide extract failed to separate the majority of this aggregate. Disaggregation of this complex was accomplished by very mild acid treatment; purification was achieved by DEAE-Sephacel anion-exchange chromatography. Polysaccharide A had a neutral charge at pH 7.3, a net negative charge at pH 8.6, and an average M(r) = 110,000; chemical analysis showed it to contain galactose, galactosamine, and an unidentified amino sugar. Polysaccharide B eluted from the anion-exchange column with increased salt concentration; it had a net negative charge and an average M(r) = 200,000, and contained fucose, galactose, quinovosamine, galacturonic acid, and glucosamine. Neither of these polysaccharides contained detectable 3-deoxy-D-manno-octolusonic acid, and both were recognized as distinct antigens on the basis of their reactivity with monoclonal antibodies CE3 and F10, which reacted with the complex before acid treatment. These data indicate that the capsule of B. fragilis NCTC 9343 comprises two discrete, surface-exposed polysaccharides with differing physicochemical properties that are distinct from the lipopolysaccharide of this organism. The finding of two surface polysaccharides has not been described for other bacteria pathogenic to humans.