The homeoproteins MAB-18 and CEH-14 insulate the dauer collagen gene col-43 from activation by the adjacent promoter of the spermatheca gene sth-1 in Caenorhabditis elegans
The homeoproteins MAB-18 and CEH-14 insulate the dauer collagen gene col-43 from activation by the adjacent promoter of the spermatheca gene sth-1 in Caenorhabditis elegans
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DOI：
10.1016/j.jmb.2005.01.045
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发表时间：
2005-04-22
影响因子：
5.6
通讯作者：
Kagawa, H
中科院分区：
文献类型：
--
作者：
Bando, T;Ikeda, T;Kagawa, H
本研究的基因组搜索表明，线虫秀丽隐杆线虫基因组有2582个双向基因，占总基因的25%以上。我们分析了这些预测的双向启动子之一的转录抑制系统，该启动子控制受精囊基因 sth-1 和胶原蛋白基因 col-43 的表达。这两个基因相距 1.3 kb，双向转录。 sth-1 在 L4 阶段后在受精囊中表达，col-43 在 L2d 阶段的皮下细胞中表达。 sth-1 和 col-43 表达所需的上游区域共享重叠的控制序列。通过酵母单杂交筛选分离出两种同源蛋白 MAB-18 和 CEH-14 作为重叠区域的结合蛋白。 MAB-18 与两个同源结构域结合位点结合，并与 CEH-14 相互作用，抑制受精囊中 col-43 的表达。这些结果表明两种同源蛋白相互作用以抑制表达 sth-1 的组织中的 col-43 表达。这是在线虫基因组中双向基因调控分析的第一份报告。 (c) 2005 Elsevier Ltd. 保留所有权利。
Genome searches in this study indicate that the nematode Caenorhabditis elegans genome has 2582 bidirectionally oriented genes that account for more than 25% of the total genes. We analyze the transcriptional repression system for one of these predicted bidirectional promoters, which controls the expression of the spermathecal gene sth-1 and collagen gene col-43. These two genes are separated by 1.3 kb and are transcribed bidirectionally. sth-1 is expressed in spermatheca after the L4 stage and col-43 is expressed in the hypodermal cells of the L2d dauer stage. The upstream regions required for the expression of sth-1 and col-43 shared an overlapped control sequence. Two homeoproteins, MAB-18 and CEH-14, were isolated by yeast one-hybrid screening as binding proteins of the overlapped region. MAB-18 bound to two homeodomain-binding sites and interacted with CEH-14 to repress col-43 expression in spermatheca. These results indicate that the two homeoproteins interact with each other to repress col-43 expression in sth-1-expressing tissues. This is the first report of bidirectional gene regulation analysis in the C. elegans genome. (c) 2005 Elsevier Ltd. All rights reserved.