First detection of a human astrovirus type 8 in a child with diarrhea in Belém, Brazil: comparison with other strains worldwide and identification of possible three lineages
First detection of a human astrovirus type 8 in a child with diarrhea in Belém, Brazil: comparison with other strains worldwide and identification of possible three lineages
复制标题
DOI：
10.1590/s0074-02762007005000032
复制
发表时间：
2007-06-01
期刊：
影响因子：
--
通讯作者：
Leite, José Paulo G
中科院分区：
文献类型：
--
作者：
Gabbay, Yvone B;Linhares, Alexandre C;Leite, José Paulo G
本研究描述了在巴西贝伦市一项基于公立医院的研究中发现的首个人类8型星状病毒（HAstV-8）的遗传关系。将该菌株与其他地方发现的具有GeneBank中可用序列的其他HAstV-8菌株进行比较。对ORF1a区（引物Mon348/Mon340）和ORF2区（引物Mon269/Mon270）进行核苷酸测序分析，发现Belem菌株与其他HAstV-8菌株具有较高的相似性。ORF1a的同源性为93 ~ 100%，ORF2的同源性为96 ~ 99%。考虑到在ORF2区观察到的序列变异（7%），我们认为HAstV-8菌株可以分为3个不同的谱系。
This study describes the genetic relationships of the first human astrovirus type-8 ( HAstV-8) detected in Belem-Brazil, during a public hospital-based study. This strain was compared with other HAstV-8 strains identified elsewhere which have sequences available at GeneBank. The regions ORF1a (primers Mon348/Mon340) and ORF2 (primers Mon269/Mon270) were analyzed by nucleotide sequencing and a high similarity rate was observed among the Belem strain and other HAstV-8 strains. In ORF1a, homology values of 93-100% were detected, and in ORF2 96-99%. Considering the sequence variation (7%) observed in ORF2 region, it was suggested that HAstV-8 strains could be divided in three different lineages.