Septin homologs cooperating in the Proliferative Stage of Microsporidia Nosema bombycis
Septin homologs cooperating in the Proliferative Stage of Microsporidia Nosema bombycis
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Septin 同源物在家蚕微孢子虫增殖阶段协同作用
DOI：
10.1016/j.jip.2021.107600
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发表时间：
2021-05-07
影响因子：
3.4
通讯作者：
Zhou，Zeyang
中科院分区：
文献类型：
--
作者：
Huang，Ju;Chen，Jie;Zhou，Zeyang
单细胞病原体家蚕微孢子虫可以感染家蚕和其他鳞翅目包括斜纹夜蛾，是第一个被鉴定的在整个生命周期中具有双核细胞核的微孢子虫。 Septin 蛋白可以形成与真菌胞质分裂相关的高度有序的丝、束或环结构。在此，描述了来自家蚕微孢子虫 CQ I 的三种 septin 蛋白（NbSeptin1、NbSeptin2 和 NbSeptin3）。这些蛋白质似乎在微孢子虫门内保守。NbSeptins 转录本在整个病原体发育周期中都被检测到，并且从感染第二天开始显着增强，这导致我们假设 NbSeptins 在生殖细胞中发挥作用。免疫荧光测定 (IFA) 显示 NbSeptins 在 meront 中广泛分布，并且 NbSeptins 部分共定位。有趣的是，在一些裂体中，NbSeptin2 和 NbSeptin3 显示出定位在双核体的细胞核之间。酵母双杂交和免疫共沉淀分析证实 NbSeptins 可以相互相互作用。我们的研究结果表明，NbSeptins 可以在家蚕微孢子虫的增殖阶段发挥作用，并有助于了解 Septins 在微孢子虫发育中的作用。
The single-celled pathogenNosema bombycis, that can infect silkwormBombyx moriand other lepidoptera including Spodoptera, is the first identified Microsporidia which has diplokaryotic nuclei throughout the life cycle. Septin proteins can form highly ordered filaments, bundles or ring structures related to the cytokinesis in fungi. Here, three septin proteins (NbSeptin1, NbSeptin2 and NbSeptin3) fromNosema bombycisCQ I are described. These proteins, appear to be conserved within the phylum Microsporidia.NbSeptinstranscripts were detected throughout the pathogen developmental cycle and were significantly enhanced from second days of infection, which lead to our hypothesis that NbSeptins play a role in merogony. Immunofluorescence assay (IFA) revealed a broad distribution of NbSeptins in meronts and partly co-localization of NbSeptins. Interestingly, in some of meronts, NbSeptin2 and NbSeptin3 showed localization between the nuclei of the diplokaryon. Yeast two-hybrid and co-immunoprecipitation analysis verified that NbSeptins can interact with each other. Our findings suggest that NbSeptins can cooperate in the proliferation stage ofNosema bombycisand contribute towards the understanding of the rols of septins in microsporidia development.