BODY COMPOSITION, AEROBIC CAPACITY, AND DENSITY OF MUSLCE CAPILLARIES IN YOUNG AND OLD MEN
BODY COMPOSITION, AEROBIC CAPACITY, AND DENSITY OF MUSLCE CAPILLARIES IN YOUNG AND OLD MEN
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DOI：
10.1152/jappl.1971.31.3.323
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发表时间：
1971-01-01
影响因子：
3.3
通讯作者：
WACHTLOV.M
中科院分区：
文献类型：
--
作者：
PARIZKOV.J;EISELT, E;WACHTLOV.M
方法受试者.一组年轻人，体育学院的学生（I）tiho没有参与系统&c顶级训练，与一组久坐不动的老年人一起进行了跟踪，根据终身体力活动分为两个亚组（表1）。亚组11，由男性谁是相当phvsicallv活跃的整个生命（至少45年）具有部分&帕特&在不同的体育活动（7，14，17-19）.第二亚组包括除了在学校和服兵役期间从未参加过任何系统体育活动的老年人。所有老年人都感到健康，并能够参加我们的实验室测试。详细的临床检查排除了有明显异常发现的受试者。亚组&，c的男性代表了一个更大的老年男性群体的一小部分，纵向跟踪了8-10年（7，17-19）。程序.通过密度测定法（即静水压称重，同时通过氮气稀释法测量肺和呼吸道中的空气）确定身体成分、ic、LBM的相对值和绝对值（6，15）。在几个老rnen身体组成的皮褶计算（17）。在自行车测功仪上研究了递增负荷过程中的最大摄氧量。根据Fischer等人的方法选择最大工作负荷。（9）采用Bergstr $ m的针吸活检技术，从股四头肌的外侧部分进行肌肉活检（4）。将组织标本立即浸入10%福尔马林中。在3-4周或更长时间的固定期后，使用经Hecht（10）修改的PAS反应。毛细血管和肌纤维的数量使用具有垂直照相机（Zeiss）的显微镜计数。从每个标本计算不同切片的20个视野。所有计算的字段大小为40，000 p2。计算毛细血管和肌纤维的横截面。毛细管：根据Krogh（13），还评估了纤维比和扩散距离（D/Z），其是两个毛细管之间的平均距离。
METHODSSubjects. A group of young men, students of the Facultv of Physical Education(I) tiho were not involved in system&c top training, was followed together with a group of old men with sedentary occupations subdivided in two subgroups according to lifelong physical activity (Table 1). Subgroup 11, consisted of men who were fairly phvsicallv active throughout life (at least 45 years) having part&pat& in different sports activities (7, 14, 17-19). Subgrouf7 II, included old men who had never engaged in any systematic sports activity, except at school and during military service. All old rnen felt healthy and were able to participate in our laboratory tests. Detailed clinical examination eliminated subjects with clearly abnormal findings. Men of subgroups &, c represented a small part of a greater group of old men followed longitudinally for 8-10 years (7, 17-19). Procedures. Body composition, ic, relative and absolute values of LBM, were ascertained by means of densitometry, ie, hydrostatic weighing with simultaneous measurement of the air in the lungs and respiratory passages by the nitrogen-dilution method (6, 15). In several old rnen body composition from skinfolds was calculated (17). The maximal oxygen uptake was investigated during a progressively increasing load on a bicycle ergometer. The maximal work load was chosen according to the method of Fischer et al.(9).Muscle biopsies were taken from the lateral part of the quadriceps muscle using the needle biopsy technique of Bergstr $ m (4). The tissue specimens were immcdiatcly im-mersed in 10% formalin. After a fixation period of 3-4 weeks or more, the PAS reaction as modified by Hecht (10) was used. The number of capillaries and muscle fibers was counted using a microscope with a vertical camera (Zeiss). Twenty fields from different sections were calculated from each specimen. The size of the field for all calculations was 40,000 p2. The capillaries and muscle fibers were calculated in cross sections. The capillary: fiber ratio and diffusion distance (D/Z) according to Krogh (13), which is the average distance between two capillaries, were also evaluated.