Shockwave Therapy Differentially Stimulates Endothelial Cells: Implications on the Control of Inflammation via Toll-Like Receptor 3
Shockwave Therapy Differentially Stimulates Endothelial Cells: Implications on the Control of Inflammation via Toll-Like Receptor 3
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DOI：
10.1007/s10753-013-9712-1
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发表时间：
2014-02-01
期刊：
影响因子：
5.1
通讯作者：
Paulus, Patrick
中科院分区：
文献类型：
--
作者：
Holfeld, Johannes;Tepekoeylue, Can;Paulus, Patrick
据报道，冲击波治疗（SWT）可改善缺血性心力衰竭的心室功能。血管生成和炎症调节作用进行了描述。然而，其机制在很大程度上仍然未知。我们假设SWT通过Toll样受体3（TLR 3）通过释放胞质RNA来调节炎症。将SWT以250脉冲、0.08 mJ/mm（2）和3 Hz施加于人脐静脉内皮细胞（HUVEC）。通过实时聚合酶链反应在不同时间点分析TLR 3、炎症基因和信号分子的基因表达。SWT显示HUVEC活化：TLR 3和核酸亲环素B的转运蛋白、促炎细胞因子亲环素A和白细胞介素-6以及抗炎白细胞介素-10的表达增强。血管内皮细胞粘附分子的表达无变化。SWT通过TLR 3途径调节炎症。在TLR 3刺激中白细胞介素（IL）-6和IL-10之间的相互作用可以示意性地视为随时间的三相调节。
Shock wave therapy (SWT) reportedly improves ventricular function in ischemic heart failure. Angiogenesis and inflammation modulatory effects were described. However, the mechanism remains largely unknown. We hypothesized that SWT modulates inflammation via toll-like receptor 3 (TLR3) through the release of cytosolic RNA. SWT was applied to human umbilical vein endothelial cells (HUVECs) with 250 impulses, 0.08 mJ/mm(2) and 3 Hz. Gene expression of TLR3, inflammatory genes and signalling molecules was analysed at different time points by real-time polymerase chain reaction. SWT showed activation of HUVECs: enhanced expression of TLR3 and of the transporter protein for nucleic acids cyclophilin B, of pro-inflammatory cytokines cyclophilin A and interleukin-6 and of anti-inflammatory interleukin-10. No changes were found in the expression of vascular endothelial cell adhesion molecule. SWT modulates inflammation via the TLR3 pathway. The interaction between interleukin (IL)-6 and IL-10 in TLR3 stimulation can be schematically seen as a three-phase regulation over time.